Although the urinary bladder urothelium has classically been thought of as a passive barrier
to ions and solutes, a number of novel properties have been recently attributed to urothelial …

… Address for reprint requests and other correspondence: LA Birder, Univ. of Pittsburgh
School of Medicine, Dept. of Medicine, A 1207 Scaife Hall, Pittsburgh, PA 15261 (e-mail: [email …

An important, but not well understood, function of epithelial cells is their ability to sense
changes in their extracellular environment and then communicate these changes to the …

In the urinary bladder, the capsaicin-gated ion channel TRPV1 is expressed both within
afferent nerve terminals and within the epithelial cells that line the bladder lumen. To determine …

Materials and Methods Reverse Transcription–PCR. Poly (A) RNA was isolated from tissue
or cultured cells by homogenization in Trizol (Life Technologies, Grand Island, NY) followed …

Mitochondrial nitric oxide synthase (mtNOS), its cellular NOS isoform, and the effects of
mitochondrially produced NO on bioenergetics have been controversial since mtNOS was first …

Nitric oxide (NO) has been implicated in the regulation of the lower urinary tract. However,
the source(s) of NO production in the urinary bladder (UB) has not been determined. …

ATP can be released from a variety of cell types by mechanical stimulation; however, the
mechanism for this release and the influence of pathology are not well understood. The present …

The umbrella cells that line the bladder are mechanosensitive, and bladder filling increases
the apical surface area of these cells; however, the upstream signals that regulate this …

We have investigated the intracellular signaling mechanisms underlying the release of nitric
oxide (NO) evoked by β-adrenoceptor (AR) agonists in urinary bladder strips and cultured …