… of reliable transformation systems for major fibrolytic rumen bacteria, (b) a poor understanding
of ecological factors that govern persistence of fibrolytic bacteria and fungi in the rumen, (c…

In the human gastrointestinal tract, dietary components, including fiber, that reach the colon
are fermented principally to short-chain fatty acids, hydrogen, and carbon dioxide. Microbial …

… Three major (Methanobrevibacter, Methanomicrobium, and Methanosphaera) and three
minor (Methanosarcina, Methanobacterium, and rumen cluster C) genera of methanogenic …

… C for 15 s and annealing temperature for 15 s, and extension at 72 C for 1 min with an
initial hold cycle of 95 C … for 60 s without final extension at 72 C for 1 min, and fluorescence …

… fed a highly tanniniferous diet (100% C. calothyrsus CPI 115690) and the isolated organisms
fell into two classes: high and low carbohydrate-fermenters (McSweeney et al., 1999). …

… Recently, temperatures in lowland tropical regions worldwide have been increasing at ≈0.25
C per decade (5) and are projected to rise by 3–8 C (mean 5 C) over the 21st century …

… : one cycle at 94C for 2 min, and 40 cycles of 94C for 30 s, 60C for 15 s and 68C for 1 min.
The … : one cycle of 50C for 2 min and 95C for 2 min for initial denaturation, and 40 cycles of 95…

… Data used for statistical analysis were the difference (C) between the temperature recorded
following the test drug and that 3 h following reserpine administration. Data were analyzed …

… Bromochloromethane reduced total methane emissions by c. 30%, with a resultant increase
in … (80–82 C) and all other methanongens (84–86 C). A decrease in the intensity of the …

BY C. MCSWEENEY, M. NEW, G. LIZCANO, AND X. LU groups outside of the major modeling
centers through the progressive phases of the Coupled-Model Intercomparison Project (…