Modulation of the phosphoinositide 3-kinase signaling pathway alters host response to sepsis, inflammation, and ischemia/reperfusion injury

DL Williams, T Ozment-Skelton, C Li - Shock, 2006 - journals.lww.com
The phosphoinositide 3-kinases (PI3Ks) are a conserved family of signal transduction enzymes
that are involved in regulating cellular activation, inflammatory responses, chemotaxis, …

Oral delivery and gastrointestinal absorption of soluble glucans stimulate increased resistance to infectious challenge

PJ Rice, EL Adams, T Ozment-Skelton… - … of Pharmacology and …, 2005 - ASPET
Glucans are immunomodulatory carbohydrates found in the cell walls of fungi and certain
bacteria. We examined the pharmacokinetics of three water-soluble glucans (glucan …

Modulation of the phosphoinositide 3-kinase pathway alters innate resistance to polymicrobial sepsis

DL Williams, C Li, T Ha, T Ozment-Skelton… - The Journal of …, 2004 - journals.aai.org
We examined the effect of modulating phosphoinositide 3-kinase (PI3K) activity in a murine
model of cecal ligation and puncture-induced polymicrobial sepsis. Inhibition of PI3K activity …

β-glucan activates microglia without inducing cytokine production in dectin-1-dependent manner

…, Y Huang, R Keshwara, T Ozment-Skelton… - The Journal of …, 2008 - journals.aai.org
… macrophages, monocytes, neutrophils, and a subset of T cells (26). Dectin-1 recognizes …
Fluorescent labeled glucans were prepared, as described by Ozment-Skelton et al. (43) and …

Vav1 and PI3K are required for phagocytosis of β-glucan and subsequent superoxide generation by microglia

VB Shah, TR Ozment-Skelton, DL Williams… - Molecular …, 2009 - Elsevier
Microglia are the resident innate immune cells that are critical for innate and adaptive
immune responses within the CNS. They recognize and are activated by pathogen-associated …

Prolonged reduction of leukocyte membrane-associated Dectin-1 levels following β-glucan administration

TR Ozment-Skelton, MP Goldman, S Gordon… - … of Pharmacology and …, 2006 - ASPET
Dectin-1 is the primary pattern recognition receptor for fungal glucans. Dectin-1 mediates
the internalization and biological response to glucans. We examined the effect of iv or ip …

Overexpression of TLR2 and TLR4 susceptibility to serum deprivation-induced apoptosis in CHO cells

W Fan, T Ha, Y Li, T Ozment-Skelton… - Biochemical and …, 2005 - Elsevier
We examined the effect of overexpression of TLR2 and TLR4 on apoptosis. TLR2 and TLR4
transfected CHO cells were subjected to serum deprivation for 0, 24, and 48h. CHO cells …

Lipopolysaccharides directly decrease Ca2+ oscillations and the hyperpolarization-activated nonselective cation current If in immortalized HL-1 cardiomyocytes

…, BM Graves, TR Ozment-Skelton… - … of Physiology-Cell …, 2010 - journals.physiology.org
Lipopolysaccharide (LPS) has been implicated in sepsis-mediated heart failure and chronic
cardiac myopathies. We determined that LPS directly and reversibly affects cardiac myocyte …

Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis

TR Ozment-Skelton, EA Defluiter, T Ha, C Li… - Critical care …, 2009 - journals.lww.com
… albicans infection decreased the percentage of T cells, it increased the percentage of splenic
T lymphocytes that express Dectin-1. The percentage of Dectin-1–positive splenic T cells …

Dectin-1 expression is altered by fungal infection, polymicrobial sepsis, and glucan administration

TR Ozment-Skelton - 2006 - search.proquest.com
Glucans are fungal cell wall PAMPs that promote survival in polymicrobial and candidal
sepsis. Dectin-1 is the primary PRR for glucans. The goals of the present study were to …