Cytochemistry and electron microscopy: the preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation

DD Sabatini, K Bensch, RJ Barrnett - The Journal of cell biology, 1963 - rupress.org
The aldehydes introduced in this paper and the more appropriate concentrations for their
general use as fixatives are: 4 to 6.5 per cent glutaraldehyde, 4 per cent glyoxal, 12.5 per cent …

Histochemical demonstration of protein-bound sulfhydryl groups

RJ Barrnett, AM Seligman - Science, 1952 - science.org
Protein Prdeei. FIG. 1. 2, 2'-dihydroxy-6, 6'-dinaphthyl disulfide (I), when used in excess at
pH 8.5, reacts with active sulfhydryl groups of fixed tissue proteins to form a colorless …

Hypothalamic lesions in goldthioglucose injected mice.

…, RJ Barrnett, J Mayer - Proceedings of the Society …, 1955 - journals.sagepub.com
Goldthioglucose injections, in the dose which produces obesity in mice, cause in these
animals extensive hypothalamic damage involving the ventromedial nuclei. Such lesions are …

The chemical nature of osmium tetroxide fixation and staining of membranes by x-ray photoelectron spectroscopy

DL White, SB Andrews, JW Faller, RJ Barrnett - Biochimica et Biophysica …, 1976 - Elsevier
X-ray photoelectron spectroscopy was used to determine the oxidation states of osmium
compounds present in erythrocyte ghost preparations and related systems treated with osmium …

Biochemical characterization and cytochemical localization of a catecholamine-sensitive adenylate cyclase in isolated capillary endothelium

RC Wagner, P Kreiner, RJ Barrnett… - Proceedings of the …, 1972 - National Acad Sciences
Capillaries were isolated from epididymal fat, and a catecholamine-sensitive adenylate
cyclase found in these capillaries was characterized. The effect of various hormones on the …

Aldehyde fixation for morphological and enzyme histochemical studies with the electron microscope

…, F Miller, RJ Barrnett - Journal of Histochemistry …, 1964 - journals.sagepub.com
Instead of discussing in detail the conditions under which fixation techniques for electron
nucroscopv can be best adapted to maintain the levels of enzymatic activity necessary for their …

Hereditary Obese-Hyperglycemic Syndrome of Mice

J Mayer, RJ Barrnett - The Yale journal of biology and medicine, 1953 - ncbi.nlm.nih.gov
METHODS Young adult mice four to five months were used in these experiments. The weight
of the obese animals was 45 to 55 g., that of the non-obese, 15 to 25 g. All animals had …

Metabolic and ultrastructural changes induced in adipose tissue by insulin

RJ Barrnett, EG Ball - The Journal of Cell Biology, 1960 - rupress.org
The addition in vitro of insulin to rat adipose tissue (epididymal) produces marked metabolic
changes which may be followed by measurement of the net gas exchange of the tissue. …

Histochemical demonstration of esterases by production of indigo

RJ Barrnett, AM Seligman - Science, 1951 - science.org
Indigo blue FIG. 1. blue is a light-fast, stable, brilliant pigment. Indoxyl butyrate was also
prepared and used in experiments to test the localization of acetyleholinesterase vs other …

Localization of lesions in the lateral hypothalamus of rats with induced adipsia and aphagia

…, RJ Barrnett, J Mayer - American Journal of …, 1958 - journals.physiology.org
A detailed examination was made of the areas damaged in animals exhibiting aphagia and
adipsia following electrolytic lesions placed in the lateral hypothalamic and sub-thalamic …