Developing MR reporter genes: promises and pitfalls

AA Gilad, PT Winnard Jr, PCM van Zijl… - NMR in Biomedicine …, 2007 - Wiley Online Library
MR reporter genes have the potential to monitor transgene expression non‐invasively in
real time at high resolution. These genes can be applied to interrogate the efficacy of gene …

RETRACTED ARTICLE: HIF-1-dependent expression of angiopoietin-like 4 and L1CAM mediates vascular metastasis of hypoxic breast cancer cells to the lungs

…, L Schito, J Chen, B Krishnamachary, PT Winnard… - Oncogene, 2012 - nature.com
Most cases of breast cancer (BrCa) mortality are due to vascular metastasis. BrCa cells must
intravasate through endothelial cells (ECs) to enter a blood vessel in the primary tumor and …

Artificial reporter gene providing MRI contrast based on proton exchange

…, MT McMahon, P Walczak, PT Winnard Jr… - Nature …, 2007 - nature.com
Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic
substances and employ water relaxation to gain contrast. We designed a nonmetallic, …

Twist Overexpression Induces In vivo Angiogenesis and Correlates with Chromosomal Instability in Breast Cancer

Y Mironchik, PT Winnard Jr, F Vesuna, Y Kato, F Wildes… - Cancer research, 2005 - AACR
Aggressive cancer phenotypes are a manifestation of many different genetic alterations that
promote rapid proliferation and metastasis. In this study, we show that stable overexpression …

Oncogenic role of DDX3 in breast cancer biogenesis

…, A Lisok, P Winnard, S Mukadam, P Van Diest… - Oncogene, 2008 - nature.com
Benzo [a] pyrene diol epoxide (BPDE), the active metabolite of benzo [a] pyrene present in
tobacco smoke, is a major cancer-causing compound. To evaluate the effects of BPDE on …

Nrf2-deficiency creates a responsive microenvironment for metastasis to the lung

…, J Takai, JM Maher, T Suzuki, PT Winnard Jr… - …, 2010 - academic.oup.com
… ( E ) Quantification of bioluminescence signal from metastatic cancer areas (* P < 0.05 by …
( E ) Quantification of bioluminescence signal from metastatic cancer areas (* P < 0.05 by …

Real time non‐invasive imaging of receptor–ligand interactions in vivo

P Winnard Jr, V Raman - Journal of cellular biochemistry, 2003 - Wiley Online Library
Non‐invasive longitudinal detection and evaluation of gene expression in living animals can
provide investigators with an understanding of the ontogeny of a gene's biological function(s…

Enhanced osteoblast response to a polymethylmethacrylate–hydroxyapatite composite

AM Moursi, AV Winnard, PL Winnard, JJ Lannutti… - Biomaterials, 2002 - Elsevier
… The results indicated that the addition of HA to the PMMA produced a significant (p<0.0001)
increase in the ultimate stress from 44±4 MPa (PMMA) to 83±11 MPa (PMMA/HA). …

Hypoxia regulates choline kinase expression through hypoxia-inducible factor-1α signaling in a human prostate cancer model

K Glunde, T Shah, PT Winnard Jr, V Raman, T Takagi… - Cancer research, 2008 - AACR
… Figure 4 shows that PC (P < 0.05) as well as tCho (P < 0.05) concentrations and the PC to
GPC ratio (P < 0.05 for wild-type and P < 0.01 for PC-3-5HRE-EGFP) significantly increased …

Molecular imaging of metastatic potential

PT Winnard, AP Pathak, S Dhara, SY Cho… - Journal of Nuclear …, 2008 - Soc Nuclear Med
If molecular imaging is to prove clinically useful it will have to surpass current, primarily
anatomic techniques in terms of sensitivity and the ability to detect minimal changes in tissue. …