A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector
pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid …

… Gentamicin sulfate was dissolved in saturated sodium bicarbonate and reacted with N,N′-methylenebisarcylamide
(1.5 eq) at 60 C for 3 days and dialyzed against water, yielding a …

Human growth hormone (HGH), somatotropin, is a protein consisting of 191 amino acids
with a molecular weight of ∼22,000. It is essential for linear growth and its application in the …

The successful cloning of the gene for human erythropoietin (EPO)(Jacobs et al. 1985; Lin
et al. 1985) has yielded information on the genetic organization and protein structure of this …

… Mature seed and whole seedlings were extracted using a modification of the protocol
described by Stebbins et al. (1991). Urea was measured as ammonia liberated from perchlorate …

Five human interferon-α (leukocyte) subtypes derived from genes cloned in Escherichia coli
have been compared for their ability to induce antiviral activity against vesicular stomatitis …

The antigrowth effects of 5 different cloned human leukocyte IFN subtypes (IFN-αA, B, C, D,
F) and 2 molecular hybrids between them (IFN-αAD(BglII) and IFN-αDA(BglII)) were …

The advent of H7N9 in early 2013 is of concern for a number of reasons, including its capability
to infect humans, the lack of clarity in the etiology of infection, and because the human …

… Each hybrid differs from the parental molecule by 13 or 16 amino acids depending on
whether the N-terminal or C-terminal fragment was derived from LeIF-A or -D. The number of …

Clonogenic tumor cells from fresh biopsies of human cancers were cultivated in vitro and
tested for sensitivity by continuous exposure to pharmacologically achievable concentrations of …