Despite significant technological and pharmacological advancements, insulin replacement
therapy fails to adequately replicate β-cell function, and so glucose control in type 1 diabetes …

The obese gene product, leptin is an important circulating satiety factor that regulates energy
balance via its actions in the hypothalamus. However, leptin receptors are also expressed …

The sulphonylureas, tolbutamide (0.1-10 mM) and glibenclamide (0.1-100 microM) shown
not to inhibit ATP-K+ channel currents when applied to inside-out membrane patches excised …

… Draining lymph node (DLN) cells (10 6 /ml) were incubated ± 50 ng/ml PMA plus 500 ng/ml
ionomycin for 1 h before addition of 10 μg/ml Brefeldin A (Sigma–Aldrich, UK) for a further 5 h …

The effects of various sulphonylureas and diazoxide on insulin secretion and the activity of
various channels have been studied using tissue culture and patch-clamp methods in an …

… PO, as described previously (Lee, Rowe & Ashford, 1995). All solution changes were
achieved by superfusing the bath with a gravity feed systemat a rate of 10 ml min-', which allowed …

OBJECTIVE AMP-activated protein kinase (AMPK) signaling acts as a sensor of nutrients
and hormones in the hypothalamus, thereby regulating whole-body energy homeostasis. …

Alloxan and its auto-oxidation product hydrogen peroxide (H2O2) irreversibly depolarize
insulinoma cells by opening a non-selective cation channel. The channel opened is …

The effects of diazoxide on ATP-K+ channel currents, recorded from the insulin-secreting
cell line, CRI-G1, were studied using patch-clamp techniques. 2. Under current-clamp …

1. We have shown, using intracellular and cell‐attached recordings, that glucose‐receptive (GR)
neurones of obese Zucker rats exhibit abnormal electrophysiological responses to …