… transactivation in HEK293 cells and rat astrocytes [85, Belcheva et al., unpubl. observations].
… 18 Coscia CJ, Bohn LM, Belcheva MM: Mechanism of kappa and mu opioid modulation of …

Acute μ and κ opioids activate the ERK/MAPK phosphorylation cascade that represents an
integral part of the signaling pathway of growth factors in astrocytes. By this cross-talk, opioids …

Phosphorylation of the MAPK isoform ERK by G protein-coupled receptors involves multiple
signaling pathways. One of these pathways entails growth factor receptor transactivation …

… renatured with buffer containing 50 mM Tris-HC1 (pH 8.0), 5 mM /3mercaptoethanol, and …
with 40 mM HEPES buffer (pH 8.0) containing 2 mM dithiothreitol and 10 mM MgCl2 at room …

… lysed with buffer containing 20 mM HEPES, 10 mM EGTA, 40 mM β-glycerophosphate, 2.5
mM MgCl 2 , 2 mM sodium vanadate, 1% Nonidet-40, 1 mM phenylmethylsulfonyl fluoride, 20 …

Growth factors, hormones, and neurotransmitters have been implicated in the regulation of
stem cell fate. Since various neural precursors express functional neurotransmitter receptors, …

… [137 mM NaCl, 20 mM Tris base (pH 7.5), 1 mM EGTA, 1 mM … : 1 mM sodium vanadate, 1
mM ZnCl 2 , 4.5 mM sodium … μg/ml aprotinin, and 1 mM phenylmethylsulfonyl fluoride. Lysates …

Opioid receptor signaling via EGF receptor (EGFR) transactivation and ERK/MAPK
phosphorylation initiates diverse cellular responses that are cell type-dependent. In astrocytes, …

… (20 mM HEPES, 10 mM EGTA, 40 mM β-glycerophosphate, 2.5 mM MgCl 2 , 2 mM sodium
… A cocktail containing 10 μg/ml leupeptin, 2 μg/ml pepstatin A, 200 μg/ml bacitracin, and 1 mM …

Previously, we implicated the opioid receptor (OR), G βγ subunits, and Ras in the opioid
activation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-…