User profiles for L. M. Needham
Lisa-Maria NeedhamDirector of CAIC and SBS Microscopy Network, University of Cambridge Verified email at cam.ac.uk Cited by 715 |
Single-molecule visualization of DNA G-quadruplex formation in live cells
Substantial evidence now exists to support that formation of DNA G-quadruplexes (G4s) is
coupled to altered gene expression. However, approaches that allow us to probe G4s in living …
coupled to altered gene expression. However, approaches that allow us to probe G4s in living …
Rational design of a conformation-specific antibody for the quantification of Aβ oligomers
…, S Chhangur, LM Needham… - Proceedings of the …, 2020 - National Acad Sciences
Protein misfolding and aggregation is the hallmark of numerous human disorders, including
Alzheimer’s disease. This process involves the formation of transient and heterogeneous …
Alzheimer’s disease. This process involves the formation of transient and heterogeneous …
[PDF][PDF] Poly (ADP-Ribose) links the DNA damage response and biomineralization
Biomineralization of the extracellular matrix is an essential, regulated process. Inappropriate
mineralization of bone and the vasculature has devastating effects on patient health, yet an …
mineralization of bone and the vasculature has devastating effects on patient health, yet an …
[HTML][HTML] ThX–a next-generation probe for the early detection of amyloid aggregates
Neurodegenerative diseases such as Alzheimer's and Parkinson's are associated with
protein misfolding and aggregation. Recent studies suggest that the small, rare and …
protein misfolding and aggregation. Recent studies suggest that the small, rare and …
Spectrally resolved photodynamics of individual emitters in large-area monolayers of hexagonal boron nitride
Hexagonal boron nitride (h-BN) is a 2D, wide band gap semiconductor that has recently
been shown to display bright room-temperature emission in the visible region, sparking …
been shown to display bright room-temperature emission in the visible region, sparking …
[HTML][HTML] Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation
…, F Chen, Y Zhang, P Flagmeier, LM Needham… - Journal of Biological …, 2021 - ASBMB
TREM2 is a pattern recognition receptor, expressed on microglia and myeloid cells, detecting
lipids and Aβ and inducing an innate immune response. Missense mutations (eg, R47H) of …
lipids and Aβ and inducing an innate immune response. Missense mutations (eg, R47H) of …
[HTML][HTML] Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD
…, J Balmer, TA Drury, AR Carr, LM Needham… - Nature structural & …, 2023 - nature.com
To understand how the nucleosome remodeling and deacetylase (NuRD) complex regulates
enhancers and enhancer–promoter interactions, we have developed an approach to …
enhancers and enhancer–promoter interactions, we have developed an approach to …
[HTML][HTML] Single-molecule fluorescence detection of a tricyclic nucleoside analogue
GN Samaan, MK Wyllie, JM Cizmic, LM Needham… - Chemical …, 2021 - pubs.rsc.org
Fluorescent nucleobase surrogates capable of Watson–Crick hydrogen bonding are
essential probes of nucleic acid structure and dynamics, but their limited brightness and short …
essential probes of nucleic acid structure and dynamics, but their limited brightness and short …
[HTML][HTML] FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
A major challenge in single-molecule imaging is tracking the dynamics of proteins or
complexes for long periods of time in the dense environments found in living cells. Here, we …
complexes for long periods of time in the dense environments found in living cells. Here, we …
A comparative photophysical study of structural modifications of Thioflavin T-inspired fluorophores
LM Needham, J Weber, CM Pearson… - The journal of …, 2020 - ACS Publications
The benzothiazolium salt, Thioflavin T (ThT), has been widely adopted as the “gold-standard”
fluorescent reporter of amyloid in vitro. Its properties as a molecular rotor result in a large-…
fluorescent reporter of amyloid in vitro. Its properties as a molecular rotor result in a large-…