Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics

HS Mchaourab, MA Lietzow, K Hideg, WL Hubbell - Biochemistry, 1996 - ACS Publications
Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled
with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the …

A novel reversible thiol-specific spin label: papain active site labeling and inhibition

LJ Berliner, J Grunwald, HO Hankovszky, K Hideg - Analytical biochemistry, 1982 - Elsevier
A new, highly reactive, thiol-specific spin label, (1-oxyl-2,2,5,5-tetramethyl-Δ 3 -pyrroline-3-methyl)methanethiosulfonate
was synthesized. Its unique specificity was demonstrated with …

Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations

C Altenbach, KJ Oh, RJ Trabanino, K Hideg… - Biochemistry, 2001 - ACS Publications
Magnetic dipolar interactions between pairs of solvent-exposed nitroxide side chains separated
by approximately one to four turns along an α-helix in T4 lysozyme are investigated. The …

Detection of singlet oxygen and superoxide with fluorescent sensors in leaves under stress by photoinhibition or UV radiation

…, T Kálai, I Vass, K Hideg, K Asada - Plant and Cell …, 2002 - academic.oup.com
In order to understand the physiological functions of reactive oxygen species (ROS)
generated in leaves, their direct measurement in vivo is of special importance. Here we report …

Molecular motion of spin labeled side chains in α-helices: analysis by variation of side chain structure

L Columbus, T Kálai, J Jekö, K Hideg, WL Hubbell - Biochemistry, 2001 - ACS Publications
K 2 CO 3 , rt 3 h, 72%; iv: NaSSO 2 CH 3 , DMSO, 30 min, 70 C, 41%. … are roughly equal
around T e,119 ≈ 297 K (24 C) and T e,131 ≈ 273 K (0 C), respectively. At these temperatures, …

Photoinhibition of photosynthesis in vivo results in singlet oxygen production detection via nitroxide-induced fluorescence quenching in broad bean leaves

É Hideg, T Kálai, K Hideg, I Vass - Biochemistry, 1998 - ACS Publications
In plants experiencing environmental stress, the formation of reactive oxygen is often presumed.
In this study, singlet oxygen was detected in broad bean (Vicia faba) leaves that were …

Site-specific incorporation of biophysical probes into proteins.

…, DR Benson, CA Altenbach, K Hideg… - Proceedings of the …, 1994 - National Acad Sciences
Biophysical probes which can detect structural changes in proteins and the interaction of
proteins with other macromolecules are important tools in studying protein function. Many …

Site-directed spin labeling of a genetically encoded unnatural amino acid

…, D Cascio, FB Peters, K Hideg… - Proceedings of the …, 2009 - National Acad Sciences
… Samples of 200 μM spin-labeled T4L containing 20% glycerol (vol/vol) were loaded into
quartz capillaries and then flash-frozen in liquid nitrogen; data were collected at 80 K. A custom …

Structure and dynamics of a conformationally constrained nitroxide side chain and applications in EPR spectroscopy

…, D Cascio, T Kálai, K Hideg… - Proceedings of the …, 2011 - National Acad Sciences
A disulfide-linked nitroxide side chain (R1) is the most widely used spin label for determining
protein topology, mapping structural changes, and characterizing nanosecond backbone …

Diphenyl Difluoroketone: A Curcumin Derivative with Potent In vivo Anticancer Activity

…, R George, P Kuppusamy, RP Ramanujam, K Hideg… - Cancer research, 2008 - AACR
Diphenyl difluoroketone (EF24), a molecule having structural similarity to curcumin, was
reported to inhibit proliferation of a variety of cancer cells in vitro. However, the efficacy and in …