Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics
HS Mchaourab, MA Lietzow, K Hideg, WL Hubbell - Biochemistry, 1996 - ACS Publications
Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled
with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the …
with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the …
A novel reversible thiol-specific spin label: papain active site labeling and inhibition
LJ Berliner, J Grunwald, HO Hankovszky, K Hideg - Analytical biochemistry, 1982 - Elsevier
A new, highly reactive, thiol-specific spin label, (1-oxyl-2,2,5,5-tetramethyl-Δ 3 -pyrroline-3-methyl)methanethiosulfonate
was synthesized. Its unique specificity was demonstrated with …
was synthesized. Its unique specificity was demonstrated with …
Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations
C Altenbach, KJ Oh, RJ Trabanino, K Hideg… - Biochemistry, 2001 - ACS Publications
Magnetic dipolar interactions between pairs of solvent-exposed nitroxide side chains separated
by approximately one to four turns along an α-helix in T4 lysozyme are investigated. The …
by approximately one to four turns along an α-helix in T4 lysozyme are investigated. The …
Detection of singlet oxygen and superoxide with fluorescent sensors in leaves under stress by photoinhibition or UV radiation
…, T Kálai, I Vass, K Hideg, K Asada - Plant and Cell …, 2002 - academic.oup.com
In order to understand the physiological functions of reactive oxygen species (ROS)
generated in leaves, their direct measurement in vivo is of special importance. Here we report …
generated in leaves, their direct measurement in vivo is of special importance. Here we report …
Molecular motion of spin labeled side chains in α-helices: analysis by variation of side chain structure
L Columbus, T Kálai, J Jekö, K Hideg, WL Hubbell - Biochemistry, 2001 - ACS Publications
… K 2 CO 3 , rt 3 h, 72%; iv: NaSSO 2 CH 3 , DMSO, 30 min, 70 C, 41%. … are roughly equal
around T e,119 ≈ 297 K (24 C) and T e,131 ≈ 273 K (0 C), respectively. At these temperatures, …
around T e,119 ≈ 297 K (24 C) and T e,131 ≈ 273 K (0 C), respectively. At these temperatures, …
Photoinhibition of photosynthesis in vivo results in singlet oxygen production detection via nitroxide-induced fluorescence quenching in broad bean leaves
In plants experiencing environmental stress, the formation of reactive oxygen is often presumed.
In this study, singlet oxygen was detected in broad bean (Vicia faba) leaves that were …
In this study, singlet oxygen was detected in broad bean (Vicia faba) leaves that were …
Site-specific incorporation of biophysical probes into proteins.
…, DR Benson, CA Altenbach, K Hideg… - Proceedings of the …, 1994 - National Acad Sciences
Biophysical probes which can detect structural changes in proteins and the interaction of
proteins with other macromolecules are important tools in studying protein function. Many …
proteins with other macromolecules are important tools in studying protein function. Many …
Site-directed spin labeling of a genetically encoded unnatural amino acid
…, D Cascio, FB Peters, K Hideg… - Proceedings of the …, 2009 - National Acad Sciences
… Samples of 200 μM spin-labeled T4L containing 20% glycerol (vol/vol) were loaded into
quartz capillaries and then flash-frozen in liquid nitrogen; data were collected at 80 K. A custom …
quartz capillaries and then flash-frozen in liquid nitrogen; data were collected at 80 K. A custom …
Structure and dynamics of a conformationally constrained nitroxide side chain and applications in EPR spectroscopy
…, D Cascio, T Kálai, K Hideg… - Proceedings of the …, 2011 - National Acad Sciences
A disulfide-linked nitroxide side chain (R1) is the most widely used spin label for determining
protein topology, mapping structural changes, and characterizing nanosecond backbone …
protein topology, mapping structural changes, and characterizing nanosecond backbone …
Diphenyl Difluoroketone: A Curcumin Derivative with Potent In vivo Anticancer Activity
…, R George, P Kuppusamy, RP Ramanujam, K Hideg… - Cancer research, 2008 - AACR
Diphenyl difluoroketone (EF24), a molecule having structural similarity to curcumin, was
reported to inhibit proliferation of a variety of cancer cells in vitro. However, the efficacy and in …
reported to inhibit proliferation of a variety of cancer cells in vitro. However, the efficacy and in …