… Clackson and JA Wells, unpublished data]; in addition, the W104A mutant has a native-like
circular dichroism spectrum (10) and can bind to a variant of hGH engineered to have higher …

… For the assay, 10 μl of the prepared protein (1 μM final) in reaction buffer was added to the
wells. To … Burlingame and J. Sadowsky for assistance with the tethering screen; P. Ren and Y. …

… Cunningham and J. Wells, personal communication), and RNase S peptide and S protein
( ) (Mitchinson & Baldwin, 1986). The dashed line represents a line of unity slope, and the …

Targeting the interfaces between proteins has huge therapeutic potential, but discovering
small-molecule drugs that disrupt protein–protein interactions is an enormous challenge. …

A strategy, called alanine-scanning mutagenesis, was used to identify specific side chains
in human growth hormone (hGH) that strongly modulate binding to the hGH receptor cloned …

Protein–protein interactions have a key role in most biological processes, and offer attractive
opportunities for therapeutic intervention. Developing small molecules that modulate protein…

Background The relative efficacy and safety of intravitreous aflibercept, bevacizumab, and
ranibizumab in the treatment of diabetic macular edema are unknown. Methods At 89 clinical …

Human growth hormone (hGH) forms a 1:2 complex with the extracellular domain of its
receptor-binding protein (hGHbp) as studied by crystallization, size exclusion chromatography, …

The past 20 years have seen many advances in our understanding of protein-protein interactions
(PPIs) and how to target them with small-molecule therapeutics. In 2004, we reviewed …

Purpose To provide 2-year results comparing anti–vascular endothelial growth factor (VEGF)
agents for center-involved diabetic macular edema (DME) using a standardized follow-up …