A new blood–brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes

S Nakagawa, MA Deli, H Kawaguchi… - Neurochemistry …, 2009 - Elsevier
Blood–brain barrier (BBB) characteristics are induced and maintained by cross-talk between
brain microvessel endothelial cells and neighbouring elements of the neurovascular unit. …

[HTML][HTML] Max is a repressor of germ cell-related gene expression in mouse embryonic stem cells

…, D Okamura, Y Tokitake, M Ikeda, H Kawaguchi… - Nature …, 2013 - nature.com
Embryonic stem cells and primordial germ cells (PGCs) express many pluripotency-associated
genes, but embryonic stem cells do not normally undergo conversion into primordial …

[HTML][HTML] Highly effective recombinant format of a humanized IgG-like bispecific antibody for cancer immunotherapy with retargeting of lymphocytes to tumor cells

R Asano, Y Watanabe, H Kawaguchi… - Journal of biological …, 2007 - ASBMB
We previously reported the marked in vitro and in vivo antitumor activity of hEx3, a humanized
diabody (small recombinant bispecific antibody) with epidermal growth factor receptor (…

Initiation of meiosis and sporulation in Saccharomyces cerevisiae requires a novel protein kinase homologue

M Yoshida, H Kawaguchi, Y Sakata… - Molecular and General …, 1990 - Springer
SME1 was cloned due to its high copy number effect: it enabled MATα/MATα diploid cells to
undergo meiosis and sporulation in a vegetative medium. Disruption of SME1 resulted in a …

Construction and humanization of a functional bispecific EGFR× CD16 diabody using a refolding system

R Asano, M Nakayama, H Kawaguchi… - The FEBS …, 2012 - Wiley Online Library
We previously reported the construction and activity of a humanized, bispecific diabody (hEx3)
that recruited T cells towards an epidermal growth factor receptor (EGFR) positive tumor. …

Diabody-based recombinant formats of humanized IgG-like bispecific antibody with effective retargeting of lymphocytes to tumor cells

R Asano, H Kawaguchi, Y Watanabe… - Journal of …, 2008 - journals.lww.com
Recently, recombinant antibodies have been dissected into antigen-binding regions and
rebuilt into multivalent high-avidity formats. These new structural designs are expected to …

[HTML][HTML] Highly enhanced cytotoxicity of a dimeric bispecific diabody, the hEx3 tetrabody

R Asano, K Ikoma, Y Sone, H Kawaguchi, S Taki… - Journal of biological …, 2010 - ASBMB
We previously reported the utility for cancer immunotherapy of a humanized bispecific diabody
(hEx3) that targets epidermal growth factor receptor and CD3. Here, we used dynamic …

Application of the Fc fusion format to generate tag‐free bi‐specific diabodies

R Asano, K Ikoma, H Kawaguchi, Y Ishiyama… - The FEBS …, 2010 - Wiley Online Library
We previously reported the use of a humanized bi‐specific diabody that targets epidermal
growth factor receptor and CD3 (hEx3‐Db) for cancer immunotherapy. Bacterial expression …

Estimation of contribution of CYP2D6 to tipepidine metabolism in humans and prolongation of the half-life of tipepidine by combination use with a CYP2D6 inhibitor in …

S Hayashi, H Kawaguchi, T Watanabe, I Miyawaki… - Xenobiotica, 2023 - Taylor & Francis
Full article: Estimation of contribution of CYP2D6 to tipepidine metabolism in humans and
prolongation of the half-life of tipepidine by combination use with a CYP2D6 inhibitor in …

Micro fluorescent analysis system integrating GaN-light-emitting-diode on a silicon platform

H Nakazato, H Kawaguchi, A Iwabuchi, K Hane - Lab on a Chip, 2012 - pubs.rsc.org
A micro fluorescent analysis system is proposed using silicon micromachining. GaN blue
light-emitting diode (LED) monolithically integrated on a silicon substrate is used as a light …