[HTML][HTML] IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector
H Mizuguchi, Z Xu, A Ishii-Watabe, E Uchida… - Molecular Therapy, 2000 - cell.com
The internal ribosome entry site (IRES) has been widely used to coexpress heterologous
gene products by a message from a single promoter. However, little is known about the …
gene products by a message from a single promoter. However, little is known about the …
Importance of neonatal FcR in regulating the serum half-life of therapeutic proteins containing the Fc domain of human IgG1: a comparative study of the affinity of …
T Suzuki, A Ishii-Watabe, M Tada… - The journal of …, 2010 - journals.aai.org
The neonatal FcR (FcRn) binds to the Fc domain of IgG at acidic pH in the endosome and
protects IgG from degradation, thereby contributing to the long serum half-life of IgG. To date, …
protects IgG from degradation, thereby contributing to the long serum half-life of IgG. To date, …
A comparison of the tube forming potentials of early and late endothelial progenitor cells
…, Q Li, T Kanayasu-Toyoda, A Ishii-Watabe… - Experimental cell …, 2008 - Elsevier
The identification of circulating endothelial progenitor cells (EPCs) has revolutionized
approaches to cell-based therapy for injured and ischemic tissues. However, the mechanisms by …
approaches to cell-based therapy for injured and ischemic tissues. However, the mechanisms by …
Optimization of transcriptional regulatory elements for constructing plasmid vectors
ZL Xu, H Mizuguchi, A Ishii-Watabe, E Uchida… - Gene, 2001 - Elsevier
In studies regarding both gene therapy and gene function, transgene expression by plasmid
vectors benefits from the use of transcriptional regulatory elements which permit high-level …
vectors benefits from the use of transcriptional regulatory elements which permit high-level …
Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells
E Uchida, H Mizuguchi, A Ishii-Watabe… - Biological and …, 2002 - jstage.jst.go.jp
MATERIALS AND METHODS Cells The human cultured cell lines and primary cells listed
below were used. HeLa, 293, A-172, CCD-14Br, HuH-7, SBC-1, NB-1, K-562, HL60, U937 and …
below were used. HeLa, 293, A-172, CCD-14Br, HuH-7, SBC-1, NB-1, K-562, HL60, U937 and …
Antibody feedback contributes to facilitating the development of Omicron-reactive memory B cells in SARS-CoV-2 mRNA vaccinees
…, T Okamoto, N Hashii, A Ishii-Watabe… - Journal of Experimental …, 2022 - rupress.org
In contrast to a second dose of the SARS-CoV-2 mRNA vaccine, a third dose elicits potent
neutralizing activity against the Omicron variant. To address the underlying mechanism for this …
neutralizing activity against the Omicron variant. To address the underlying mechanism for this …
A central role for nicotinic cholinergic regulation of growth factor–induced endothelial cell migration
…, R Katzenberg-Clark, A Ishii-Watabe… - … , and vascular biology, 2007 - Am Heart Assoc
Objective— An endothelial nicotinic acetylcholine receptor (nAChR) participates in
atherogenesis and tumorigenesis by promoting neovascularization. To date, the mechanisms of …
atherogenesis and tumorigenesis by promoting neovascularization. To date, the mechanisms of …
Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies
…, M Tada, M Kiyoshi, A Matsuda, A Ishii-Watabe - MAbs, 2019 - Taylor & Francis
Typical crystallizable fragment (Fc) glycans attached to the CH2 domain in therapeutic
monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans…
monoclonal antibodies (mAbs) are core-fucosylated and asialo-biantennary complex-type glycans…
Glycosylation of IgG-Fc: a molecular perspective
M Kiyoshi, K Tsumoto, A Ishii-Watabe… - International …, 2017 - academic.oup.com
Antibodies of the IgG class carry a pair of oligosaccharides (N-glycans) in the Fc region. The
importance of the N-glycan is clearly demonstrated by its profound effect in the …
importance of the N-glycan is clearly demonstrated by its profound effect in the …
[HTML][HTML] Assessing the heterogeneity of the Fc-glycan of a therapeutic antibody using an engineered FcγReceptor IIIa-immobilized column
…, S Oe, T Ide, K Tsumoto, A Ishii-Watabe - Scientific reports, 2018 - nature.com
The N-glycan moiety of IgG-Fc has a significant impact on multifaceted properties of antibodies
such as in their effector function, structure, and stability. Numerous studies have been …
such as in their effector function, structure, and stability. Numerous studies have been …