[PDF][PDF] The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation
V Tripathi, JD Ellis, Z Shen, DY Song, Q Pan, AT Watt… - Molecular cell, 2010 - cell.com
… Similarly, PSP1, an RNA-binding protein and a component of paraspeckles, also displayed
a weak interaction with MALAT1 but at the same time showed a strong association with the …
a weak interaction with MALAT1 but at the same time showed a strong association with the …
Targeted degradation of sense and antisense C9orf72 RNA foci as therapy for ALS and frontotemporal degeneration
… in the first intron of RNAs initiated at the 5′-most transcription initiation … Error bars represent
SE (SEM) from at least three … 4 F and G; P > 0.24 and P > 0.12 by t test at any time point for …
SE (SEM) from at least three … 4 F and G; P > 0.24 and P > 0.12 by t test at any time point for …
Analgesic profile of intrathecal P2X3 antisense oligonucleotide treatment in chronic inflammatory and neuropathic pain states in rats
P Honore, K Kage, J Mikusa, AT Watt, JF Johnston… - Pain, 2002 - Elsevier
… Although P2X 3 antisense treatment was stopped at day 7, the anti-allodynic effects of P2X
3 antisense treatment were still observed at day 9 (51.7±12.4% reduction, P<0.05) and day …
3 antisense treatment were still observed at day 9 (51.7±12.4% reduction, P<0.05) and day …
Analysis of the androgen receptor–regulated lncRNA landscape identifies a role for ARLNC1 in prostate cancer progression
…, R Backofen, CS Sahinalp, SM Freier, AT Watt… - Nature …, 2018 - nature.com
… , increased levels of AR binding at transcription start sites (… of BRD4 and AR proteins at the
promoters of direct AR-… genes, noncoding ARGs were detected at lower overall levels (Fig. 1c)…
promoters of direct AR-… genes, noncoding ARGs were detected at lower overall levels (Fig. 1c)…
Rational design of antisense oligonucleotides targeting single nucleotide polymorphisms for potent and allele selective suppression of mutant Huntingtin in the CNS
…, AL Southwell, H Kordasiewicz, AT Watt… - Nucleic acids …, 2013 - academic.oup.com
… The labeling reaction was incubated at 37C for 30 min followed by heating at 90C for 1
min. Labeled RNA was purified using 12% denaturing polyacrylamide gel. ASO (200 nM), …
min. Labeled RNA was purified using 12% denaturing polyacrylamide gel. ASO (200 nM), …
[HTML][HTML] In vivo evaluation of candidate allele-specific mutant huntingtin gene silencing antisense oligonucleotides
…, NH Skotte, HB Kordasiewicz, ME Østergaard, AT Watt… - Molecular Therapy, 2014 - cell.com
… We have identified four well-tolerated lead ASOs that potently and selectively silence muHTT
at a broad range of doses throughout the central nervous system for 16 weeks or more after …
at a broad range of doses throughout the central nervous system for 16 weeks or more after …
[HTML][HTML] Allele-specific suppression of mutant huntingtin using antisense oligonucleotides: providing a therapeutic option for all Huntington disease patients
…, E Petoukhov, K Vaid, H Kordasiewicz, AT Watt… - PloS one, 2014 - journals.plos.org
… We treated primary neurons with our four lead ASO candidates at concentrations of up to
10,000 nM (Figure 5B). At the highest dose we observed spectrin cleavage just above …
10,000 nM (Figure 5B). At the highest dose we observed spectrin cleavage just above …
An exocyclic methylene group acts as a bioisostere of the 2′-oxygen atom in LNA
…, PS Pallan, H Gaus, TP Prakash, AT Watt… - Journal of the …, 2010 - ACS Publications
… At the time we initiated our synthesis program there were no literature reports describing the
… −carbon bonds by generating and trapping a radical at the 2′ position of a nucleoside with …
… −carbon bonds by generating and trapping a radical at the 2′ position of a nucleoside with …
[HTML][HTML] Antisense oligonucleotide treatment ameliorates IFN-γ–induced proteinuria in APOL1-transgenic mice
…, J Hartleib, S Kuntz, D Gattis, C Ahlström, AT Watt… - JCI insight, 2019 - ncbi.nlm.nih.gov
… African Americans develop end-stage renal disease at a higher rate compared with … ,
resulting in expression in similar tissues and at similar relative levels as humans. While naive …
resulting in expression in similar tissues and at similar relative levels as humans. While naive …
[HTML][HTML] Control of RNA processing by a large non-coding RNA over-expressed in carcinomas
R Lin, M Roychowdhury-Saha, C Black, AT Watt… - FEBS letters, 2011 - Elsevier
… 2000 for HUVECs) at 37 C for 4 h, at which time the medium was replaced with standard
growth medium. Untreated control (UTC) or scrambled ASO (Con) at 75 nM were used as …
growth medium. Untreated control (UTC) or scrambled ASO (Con) at 75 nM were used as …