Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain

Cell. 1998 May 29;93(5):717-29. doi: 10.1016/s0092-8674(00)81434-8.

Abstract

The generation of pacemaker activity in heart and brain is mediated by hyperpolarization-activated cation channels that are directly regulated by cyclic nucleotides. We previously cloned a novel member of the voltage-gated K channel family from mouse brain (mBCNG-1) that contained a carboxy-terminal cyclic nucleotide-binding domain (Santoro et al., 1997) and hence proposed it to be a candidate gene for pacemaker channels. Heterologous expression of mBCNG-1 demonstrates that it does indeed code for a channel with properties indistinguishable from pacemaker channels in brain and similar to those in heart. Three additional mouse genes and two human genes closely related to mBCNG-1 display unique patterns of mRNA expression in different tissues, including brain and heart, demonstrating that these channels constitute a widely expressed gene family.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Barium / pharmacology
  • Biological Clocks / genetics*
  • Brain / physiology*
  • Cesium / pharmacology
  • Cloning, Molecular
  • Cyclic AMP / pharmacology
  • Cyclic Nucleotide-Gated Cation Channels
  • DNA, Complementary / genetics
  • Electric Conductivity
  • Gene Expression
  • Heart / physiology
  • Humans
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
  • Ion Channels / antagonists & inhibitors
  • Ion Channels / biosynthesis
  • Ion Channels / genetics*
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • Nerve Tissue Proteins / antagonists & inhibitors
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics*
  • Neuroglia / metabolism
  • Oocytes
  • Pacemaker, Artificial
  • Patch-Clamp Techniques
  • Potassium / metabolism
  • Potassium Channels
  • Recombinant Proteins / biosynthesis
  • Sequence Homology, Amino Acid
  • Sodium / metabolism
  • Species Specificity
  • Tissue Distribution
  • Xenopus

Substances

  • Cyclic Nucleotide-Gated Cation Channels
  • DNA, Complementary
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
  • Ion Channels
  • Nerve Tissue Proteins
  • Potassium Channels
  • Recombinant Proteins
  • Cesium
  • Barium
  • Sodium
  • Cyclic AMP
  • Potassium

Associated data

  • GENBANK/AF064873
  • GENBANK/AF064874
  • GENBANK/AF064875
  • GENBANK/AF064876
  • GENBANK/AF064877