Abstract
Effects of riluzole on high voltage-activated (HVA) calcium channels of rat dorsal root ganglion neurons were studied using the whole-cell patch-clamp technique. Riluzole at 30 microM inhibited the HVA currents. The onset and offset of riluzole inhibitory effect were slow usually taking more than 3 min. Riluzole inhibition of the HVA currents was abolished and partially reduced by addition of 500 microM GDP-beta-S and 1 mM N-ethylmaleimide, respectively, to the pipette solution. Pre-treatment with pertussis toxin or application of depolarizing pre-pulses did not affect riluzole's inhibitory effect on the HVA currents. Riluzole inhibition of the HVA currents was also blocked by internal application of 50 microg/ml protein kinase A inhibitory peptide. It was concluded that pertussis toxin-insensitive G-proteins and protein kinase A may be involved in riluzole inhibition of the HVA currents.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Calcium Channels / physiology*
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Cyclic AMP-Dependent Protein Kinases / metabolism
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Enzyme Inhibitors / pharmacology
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Ethylmaleimide / pharmacology
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GTP-Binding Proteins / metabolism*
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Ganglia, Spinal / cytology
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Guanosine Diphosphate / analogs & derivatives
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Guanosine Diphosphate / pharmacology
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Ion Channel Gating / drug effects
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Neurons / chemistry*
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Neurons / drug effects
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Neurons / enzymology
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Neuroprotective Agents / pharmacology*
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Patch-Clamp Techniques
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Pertussis Toxin
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Rats
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Rats, Sprague-Dawley
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Riluzole
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Thiazoles / pharmacology*
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Thionucleotides / pharmacology
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Virulence Factors, Bordetella / pharmacology
Substances
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Calcium Channels
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Enzyme Inhibitors
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Neuroprotective Agents
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Thiazoles
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Thionucleotides
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Virulence Factors, Bordetella
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Guanosine Diphosphate
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guanosine 5'-O-(2-thiodiphosphate)
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Riluzole
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Pertussis Toxin
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Cyclic AMP-Dependent Protein Kinases
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GTP-Binding Proteins
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Ethylmaleimide