Peroxynitrite (PN) can be formed under mainly pathophysiological conditions from nitric oxide (NO) and superoxide anion and may be responsible for oxidative modifications of biomolecules. Preparations of nitric oxide synthases from porcine cerebellum (nNOS), bovine aortic endothelium (eNOS) and cytokine-treated murine macrophages (iNOS) were inhibited by PN in their ability to transform arginine to citrulline and nitric oxide with IC50 values of 15, 28, and 10 microM, respectively. Glutathione, bovine serum albumin and tyrosine provided varying degrees of protection in the three preparations. Intact endothelial cells, upon exposure to PN, rapidly lost their glutathione content but protein-SH groups and eNOS activity remained largely unaffected. Destruction of the heme-thiolate catalytic site was observed when nNOS was exposed to PN suggesting that the irreversible oxidation of this bond may be the common mechanism of NOS inhibition.