Abstract
Vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) proliferate faster and are more sensitive to transforming growth factor-beta 1 (TGF-beta 1) than those of normotensive Wistar-Kyoto rats. We studied the in vitro effects of tranilast, an anti-allergic drug, on the proliferation, migration and extracellular matrix synthesis in the SHR-VSMC. There were many inhibitory effects of tranilast (30-300 microM) on SHR-VSMC. One is the effect on the proliferation stimulated with fetal bovine serum (FBS), TGF-beta 1 and platelet-derived growth factor-BB (PDGF-BB). Another is the effect on the PDGF-BB-induced migration. Lastly, tranilast exhibited inhibitory effects on spontaneous collagen synthesis and TGF-beta 1-induced collagen and glycosaminoglycan synthesis. On the other hand, collagen induced the VSMC migration concentration-dependently. These results suggest that tranilast may prevent restenosis after percutaneous transluminal coronary angioplasty.
MeSH terms
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Angioplasty, Balloon, Coronary / adverse effects
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Arterial Occlusive Diseases / prevention & control*
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Arterial Occlusive Diseases / therapy
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Cell Division / drug effects
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Chemotaxis, Leukocyte / drug effects
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Collagen / biosynthesis*
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Drug Evaluation, Preclinical
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Endothelium, Vascular / injuries
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Extracellular Matrix / metabolism
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Glycosaminoglycans / biosynthesis
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Humans
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Male
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Muscle, Smooth, Vascular / drug effects*
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Muscle, Smooth, Vascular / metabolism
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Platelet-Derived Growth Factor / antagonists & inhibitors*
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Platelet-Derived Growth Factor / pharmacology
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Rats
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Rats, Inbred SHR
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Rats, Inbred WKY
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Recombinant Proteins / antagonists & inhibitors
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Recombinant Proteins / pharmacology
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Recurrence
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Transforming Growth Factor beta / antagonists & inhibitors*
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Transforming Growth Factor beta / pharmacology
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ortho-Aminobenzoates / pharmacology*
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Glycosaminoglycans
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Platelet-Derived Growth Factor
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Recombinant Proteins
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Transforming Growth Factor beta
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ortho-Aminobenzoates
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Collagen
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tranilast