Abstract
A cDNA encoding variant form of the A3 adenosine (Ado) receptor was isolated from rat by reverse transcription of brain mRNA followed by PCR. The full-length receptor (A3i) cDNA encodes 337 amino acids and shares complete sequence identity with the rat A3 Ado receptor, except for the presence of a seventeen amino acid insert located in the second intracellular domain. In contrast to the rat A3 receptor, stable expression of A3i in CHO cells resulted in poor coupling to Gi proteins. Analysis of receptor transcripts by RT-PCR suggests that the A3 Ado receptor mRNAs are products of alternative splicing. Sequence analysis of A3 genomic DNA identified a 1.7 kb intron that is likely alternatively spliced to produce the A3 and A3i receptors.
MeSH terms
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Adenosine / analogs & derivatives
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Adenosine / pharmacology
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Alternative Splicing*
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain Chemistry
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CHO Cells
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Cell Membrane / chemistry
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Cloning, Molecular*
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Cricetinae
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Cyclic AMP / biosynthesis
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DNA, Complementary / genetics*
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Gene Expression
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Genetic Variation / genetics
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Iodobenzenes / pharmacology
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Molecular Sequence Data
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Purinergic P1 Receptor Agonists
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RNA, Messenger / analysis
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RNA, Messenger / genetics*
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Rats
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Receptors, Purinergic P1 / biosynthesis
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Receptors, Purinergic P1 / genetics*
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Spleen / chemistry
Substances
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DNA, Complementary
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Iodobenzenes
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Purinergic P1 Receptor Agonists
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RNA, Messenger
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Receptors, Purinergic P1
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3-iodo-N(6)-4-aminobenzyladenosine
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Cyclic AMP
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Adenosine