Abstract
We have cloned human alpha 1C-adrenergic receptor from human prostate cDNA library. The deduced amino acid sequence of the clone (P2C4) encodes a protein of 466 amino acids that showed strong sequence homology to the previously cloned bovine alpha 1C-adrenergic receptor. The radioligand binding properties of P2C4 expressed in COS-7 cells were very similar to those of bovine alpha 1C-adrenergic receptor. With reverse-transcription polymerase chain reaction assay, we observed alpha 1C-adrenergic receptor transcripts in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. The data show that the clone P2C4 encodes a human alpha 1C-adrenergic receptor cDNA, and the receptor subtype is expressed not widely but localized in several human tissues.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cattle
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Cell Line
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Conserved Sequence
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DNA / genetics
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DNA / metabolism
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Gene Expression
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Gene Library
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Humans
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Male
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Organ Specificity
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Polymerase Chain Reaction
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Prostate / metabolism*
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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RNA, Messenger / metabolism
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Radioligand Assay
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Receptors, Adrenergic, alpha / biosynthesis*
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Receptors, Adrenergic, alpha / genetics
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Receptors, Adrenergic, alpha / metabolism
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Restriction Mapping
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Sequence Homology, Amino Acid
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Transfection
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Adrenergic, alpha
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Recombinant Proteins
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DNA
Associated data
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GENBANK/D25235
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GENBANK/L14791
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GENBANK/L14792
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GENBANK/L14793
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GENBANK/L14794
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GENBANK/L14795
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GENBANK/L14796
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GENBANK/L14797
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GENBANK/L14798
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GENBANK/U03866