Abstract
The effect of amiloride on Na+/Ca2+ exchange activity and adenylate energy charge in isolated rat cardiac myocytes has been studied. Amiloride inhibits Na+/Ca2+ exchange activity in rat cardiac myocytes with an IC50 of 1.76 mM when added to the external side of the cell membrane, whereas it is a weaker inhibitor when present at the cytoplasmic side. The adenylate energy charge in rat cardiac myocytes is unaffected by amiloride.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Monophosphate / metabolism
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Amiloride / pharmacokinetics
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Amiloride / pharmacology*
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Animals
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Carrier Proteins / antagonists & inhibitors*
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Carrier Proteins / drug effects
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Carrier Proteins / metabolism
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Energy Metabolism
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Heart / drug effects*
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Intracellular Fluid / metabolism
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Kinetics
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Male
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Myocardium / cytology
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Myocardium / metabolism*
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Rats
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Rats, Wistar
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Sarcolemma / drug effects
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Sarcolemma / metabolism
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Sodium-Calcium Exchanger
Substances
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Carrier Proteins
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Sodium-Calcium Exchanger
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Adenosine Monophosphate
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Amiloride