Abstract
Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was blocked by naltrindol, a delta-specific antagonist. The delta-agonist evoked no or very weak currents in oocytes with the alpha subunit of Gq or G(o). These findings indicate the functional coupling between the opioid delta-receptor and phospholipase C through an activation of Gi.
MeSH terms
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Analgesics / pharmacology*
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Animals
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Benzeneacetamides*
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Electric Conductivity / drug effects
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Enkephalin, D-Penicillamine (2,5)-
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Enkephalin, Leucine / analogs & derivatives
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Enkephalin, Leucine / pharmacology
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Enkephalins / pharmacology
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Enzyme Activation
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Female
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GTP-Binding Proteins / metabolism*
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Gene Expression
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Membrane Potentials / drug effects
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Mice
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Naltrexone / analogs & derivatives
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Naltrexone / pharmacology
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Narcotic Antagonists / pharmacology
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Oocytes / drug effects
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Oocytes / metabolism
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Oocytes / physiology*
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Pyrrolidines / pharmacology
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Receptors, Opioid, delta / biosynthesis
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Receptors, Opioid, delta / drug effects
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Receptors, Opioid, delta / physiology*
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Type C Phospholipases / metabolism*
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Xenopus laevis
Substances
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Analgesics
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Benzeneacetamides
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Enkephalins
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Narcotic Antagonists
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Pyrrolidines
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Receptors, Opioid, delta
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Enkephalin, Leucine
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Naltrexone
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enkephalin, Ser(2), Leu(5), Thr(6)-
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Enkephalin, D-Penicillamine (2,5)-
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Type C Phospholipases
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GTP-Binding Proteins
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naltrindole
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U 69593