A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins

J Rouse; P Cohen; S Trigon; M Morange; A Alonso-Llamazares; D Zamanillo; T Hunt; A R Nebreda

doi:10.1016/0092-8674(94)90277-1

A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins

Cell. 1994 Sep 23;78(6):1027-37. doi: 10.1016/0092-8674(94)90277-1.

Authors

J Rouse¹, P Cohen, S Trigon, M Morange, A Alonso-Llamazares, D Zamanillo, T Hunt, A R Nebreda

Affiliation

¹ Department of Biochemistry, University of Dundee, Scotland.

PMID: 7923353
DOI: 10.1016/0092-8674(94)90277-1

Abstract

MAPK-activated protein kinase-2 (MAPKAP kinase-2) is activated in vitro by the p42 and p44 isoforms of MAPK (p42/p44MAPK). In several cell lines, however, MAPKAP kinase-2 is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that activate p42/p44MAPK. We have identified a MAPK-like enzyme that acts as a MAPKAP kinase-2 reactivating kinase (RK). RK is recognized by an antiserum raised against a Xenopus MAPK (Mpk2), which is most similar to HOG1 from S. cerevisiae. We also identified a RK kinase (RKK) on the basis of its ability to activate either RK or a GST-Mpk2 fusion protein. The RKK, RK, and MAPKAP kinase-2 constitute a new stress-activated signal transduction pathway in vertebrates that is distinct from the classical MAPK cascade.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Arsenites / pharmacology
Base Sequence
Calcium-Calmodulin-Dependent Protein Kinases / genetics
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
HSP27 Heat-Shock Proteins
Heat-Shock Proteins / metabolism*
Hot Temperature / adverse effects
Humans
Intracellular Signaling Peptides and Proteins
Mitogen-Activated Protein Kinases*
Molecular Chaperones
Molecular Sequence Data
Neoplasm Proteins / metabolism
Phosphorylation
Protein Kinases*
Protein Serine-Threonine Kinases / metabolism*
Rats
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins*
Sequence Homology, Amino Acid
Signal Transduction*
Xenopus / genetics
Xenopus Proteins*

Substances

Arsenites
HSP27 Heat-Shock Proteins
HSPB1 protein, human
Heat-Shock Proteins
Hspb1 protein, rat
Intracellular Signaling Peptides and Proteins
Molecular Chaperones
Neoplasm Proteins
Saccharomyces cerevisiae Proteins
Xenopus Proteins
Protein Kinases
MAP-kinase-activated kinase 2
Mpk2 protein, Xenopus
Protein Serine-Threonine Kinases
Calcium-Calmodulin-Dependent Protein Kinases
HOG1 protein, S cerevisiae
Mitogen-Activated Protein Kinases
mapk14a protein, Xenopus
arsenite

Associated data

GENBANK/X80751