Abstract
Suramin (1-100 microM) and alpha, beta-methylene adenosine 5'-triphosphate (AMPCPP, 39 microM), antagonized the motor activity induced by exogenous adenosine 5'-triphosphate (ATP) but not exogenous noradrenaline (NA) in the longitudinal musculature of prostatic (P) and epididymal (E) segments of the rat vas deferens. Likewise, application of these drugs reduced the fast component of the nerve-stimulated contraction in response to a single transmural electrical pulse in E and P. Suramin also blocked in a concentration-dependent fashion, the contractile responses to trains of 1.5, 5, 15 or 30 Hz transmural electrical pulses in P, while it did not affect those in E. AMPCPP obliterated responses to trains of 1.5, 5, and 15 Hz in P, while reducing these responses in E to a significantly lesser extent. Present results strongly support that ATP is the motor transmitter in P, while in E, ATP and NA are likely the co-transmitters responsible for the motor tone.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Triphosphate / analogs & derivatives
-
Adenosine Triphosphate / antagonists & inhibitors
-
Adenosine Triphosphate / pharmacology
-
Adenosine Triphosphate / physiology*
-
Animals
-
Electric Stimulation
-
Epididymis / innervation
-
Epididymis / physiology
-
In Vitro Techniques
-
Male
-
Muscle, Smooth / drug effects
-
Muscle, Smooth / innervation*
-
Muscle, Smooth / physiology
-
Neurotransmitter Agents / physiology*
-
Norepinephrine / antagonists & inhibitors
-
Norepinephrine / pharmacology
-
Prazosin / pharmacology
-
Prostate / innervation
-
Prostate / physiology
-
Rats
-
Rats, Sprague-Dawley
-
Receptors, Purinergic P2 / drug effects
-
Suramin / pharmacology
-
Vas Deferens / drug effects
-
Vas Deferens / innervation*
-
Vas Deferens / physiology
Substances
-
Neurotransmitter Agents
-
Receptors, Purinergic P2
-
5'-adenylyl (beta,gamma-methylene)diphosphonate
-
Suramin
-
Adenosine Triphosphate
-
alpha,beta-methyleneadenosine 5'-triphosphate
-
Norepinephrine
-
Prazosin