Abstract
Cortical synaptosomes were prepared from pregnant dams (GD-17) and rat fetuses (ED-17), loaded with [3H]5-HT and assayed to evaluate release mediated by cocaine (COC), fenfluramine (FEN) and 3,4-methylenedioxymethamphetamine (MDMA). COC and FEN elicited a high-affinity (10(-9) M) release response in fetal tissue which was not apparent in the dam. MDMA-induced release was similar in magnitude in both tissue types. Consequently, the release of 5-HT from developing neurons may be one mechanism by which COC and FEN elicit their teratogenetic effects in utero.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3,4-Methylenedioxyamphetamine / analogs & derivatives
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3,4-Methylenedioxyamphetamine / pharmacokinetics
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3,4-Methylenedioxyamphetamine / pharmacology
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Animals
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Brain / embryology*
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Brain Chemistry / drug effects*
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Cocaine / pharmacokinetics
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Cocaine / pharmacology
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Designer Drugs / pharmacokinetics
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Designer Drugs / pharmacology
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Female
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Fenfluramine / pharmacokinetics
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Fenfluramine / pharmacology
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In Vitro Techniques
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Maternal-Fetal Exchange
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N-Methyl-3,4-methylenedioxyamphetamine
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Potassium Chloride / pharmacology
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Pregnancy
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Rats
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Rats, Sprague-Dawley
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Serotonin / metabolism*
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Substance-Related Disorders / metabolism*
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Synaptosomes / drug effects
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Synaptosomes / metabolism
Substances
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Designer Drugs
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Fenfluramine
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Serotonin
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3,4-Methylenedioxyamphetamine
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Potassium Chloride
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Cocaine
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N-Methyl-3,4-methylenedioxyamphetamine