Abstract
Effect of potassium channel openers on membrane potential of rat liver mitochondria was studied. It has been found that potassium channel opener RP 66471 induces depolarization of the mitochondrial membrane. Since neither the inhibition of mitochondrial respiration nor the uncoupling of mitochondria was observed concomitantly, the specific effect on the mitochondrial potential is postulated. Most likely the effect is caused by the increase of permeability of the inner mitochondrial membrane to potassium ions. Interestingly, however, it was found that no other potassium channel openers tested but RP 66471 was able to induce depolarization of mitochondrial membrane.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antihypertensive Agents / pharmacology
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Benzoates / pharmacology*
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Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
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Fluoresceins
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Fluorescent Dyes
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Hydrogen-Ion Concentration
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Intracellular Membranes / drug effects
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Intracellular Membranes / physiology*
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Ion Channel Gating / drug effects
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Kinetics
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Light
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Lipid Bilayers
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Membrane Potentials / drug effects
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Mitochondria, Liver / drug effects
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Mitochondria, Liver / physiology*
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Niacinamide / analogs & derivatives
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Niacinamide / pharmacology
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Nicorandil
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Potassium / metabolism
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Potassium Channels / drug effects
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Potassium Channels / physiology*
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Pyridines / pharmacology*
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Rats
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Scattering, Radiation
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Valinomycin / pharmacology
Substances
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Antihypertensive Agents
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Benzoates
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Fluoresceins
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Fluorescent Dyes
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Lipid Bilayers
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Potassium Channels
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Pyridines
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RP 66471
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Valinomycin
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Niacinamide
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Nicorandil
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Carbonyl Cyanide m-Chlorophenyl Hydrazone
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2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein
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Potassium