Abstract
Interleukin 4 (IL-4) is a pleotropic cytokine affecting a wide range of cell types in both the mouse and the human. These activities include regulation of the growth and differentiation of both T and B lymphocytes. The activities of IL-4 in nonprimate, nonmurine systems are not well established. Herein, we demonstrate in the bovine system that IL-4 upregulates production of IgM, IgG1, and IgE in the presence of a variety of costimulators including anti-IgM, Staphylococcus aureus cowan strain I, and pokeweed mitogen. IgE responses are potentiated by the addition of IL-2 to IL-4. Culture of bovine B lymphocytes with IL-4 in the absence of additional costimulators resulted in the increased surface expression of CD23 (low-affinity Fc epsilon RII), IgM, IL-2R, and MHC class II in a dose-dependent manner. IL-4 alone increased basal levels of proliferation of bulk peripheral blood mononuclear cells but in the presence of Con A inhibited proliferation. In contrast to the activities of IL-4 in the murine system, proliferation of TH1- and TH2-like clones was inhibited in a dose-dependent manner as assessed by antigen-or IL-2-driven in vitro proliferative responses. These observations are consistent with the role of IL-4 as a key player in regulation of both T and B cell responses.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibody Formation / drug effects
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B-Lymphocytes / cytology*
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B-Lymphocytes / drug effects
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B-Lymphocytes / immunology*
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Babesia bovis / immunology
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Cattle
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Cell Differentiation / drug effects
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Cell Line
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DNA, Complementary / biosynthesis
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Fasciola hepatica / immunology
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Histocompatibility Antigens Class II / biosynthesis
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Histocompatibility Antigens Class II / drug effects
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Immunoglobulin E / biosynthesis
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Immunoglobulin E / drug effects
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Immunoglobulin G / biosynthesis
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Immunoglobulin G / drug effects
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Immunoglobulin M / biosynthesis
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Immunoglobulin M / drug effects
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Interleukin-4 / biosynthesis*
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Interleukin-4 / genetics
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Interleukin-4 / pharmacology*
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Lymphocyte Activation / drug effects
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Receptors, IgE / biosynthesis
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Receptors, IgE / drug effects
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Receptors, Interleukin-2 / biosynthesis
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Receptors, Interleukin-2 / drug effects
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Recombinant Proteins / pharmacology
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T-Lymphocytes / cytology*
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology*
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Th1 Cells / drug effects
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Th2 Cells / drug effects
Substances
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DNA, Complementary
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Histocompatibility Antigens Class II
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Immunoglobulin G
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Immunoglobulin M
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Receptors, IgE
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Receptors, Interleukin-2
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Recombinant Proteins
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Interleukin-4
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Immunoglobulin E