Abstract
Quantitative autoradiography was used to evaluate the pharmacological profile of dopamine D2-like receptors labeled by [125I]iodosulpiride. Caudate/putamen, a brain region associated primarily with dopamine D2 receptor mRNA, was used as a prototypical D2 tissue; cerebellar lobule X (D3 mRNA associated), as a D3 tissue. 7-OH-DPAT ((+/-)-2-dipropylamino-7-hydroxy-1,2,3,4- tetrahydronaphthalene) exhibited selectively for cerebellar receptors (24-fold), followed by quinpirole (6-fold). Haloperidol and domperidone were 4- and 18-fold more potent at striatal receptors, respectively. These data are in close agreement with that derived from dopamine D2 and D3 receptor-expressing cell lines.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoradiography
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Binding, Competitive
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Brain / ultrastructure*
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CHO Cells / ultrastructure
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Caudate Nucleus / ultrastructure
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Cerebellum / ultrastructure
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Cricetinae
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Dopamine Agents / metabolism*
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Iodine Radioisotopes
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Male
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Putamen / ultrastructure
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Rats
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Rats, Sprague-Dawley
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Receptors, Dopamine / metabolism*
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Receptors, Dopamine D2 / metabolism*
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Receptors, Dopamine D3
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Sensitivity and Specificity
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Sulpiride / analogs & derivatives
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Sulpiride / metabolism
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Tetrahydronaphthalenes / metabolism
Substances
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Dopamine Agents
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Drd3 protein, rat
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Iodine Radioisotopes
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Receptors, Dopamine
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Receptors, Dopamine D2
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Receptors, Dopamine D3
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Tetrahydronaphthalenes
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iodosulpride
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Sulpiride
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7-hydroxy-2-N,N-dipropylaminotetralin