Abstract
Nerve growth factor (NGF) stimulates 5-HT3 receptor expression although the mechanism(s) responsible for this effect are unknown. We report the nucleotide and deduced amino acid sequences of a nearly complete rat 5-HT3 receptor subunit and use the sequence to develop a reverse transcription-polymerase chain reaction (RT-PCR) assay that measures 5-HT3 mRNA. Exposure of PC12 cells to NGF results in increasing steady-state levels of 5-HT3 mRNA. The four-fold increase in mRNA correlates with and is sufficient to account for increases in receptor measured by agonist induction of whole cell currents.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Differentiation / drug effects
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Cloning, Molecular
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DNA, Complementary / genetics*
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Gene Expression Regulation / drug effects*
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Molecular Sequence Data
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics
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Nerve Growth Factors / pharmacology*
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / genetics
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PC12 Cells / drug effects
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PC12 Cells / metabolism
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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RNA, Neoplasm / biosynthesis
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RNA, Neoplasm / genetics
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RNA-Directed DNA Polymerase
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Rats
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Receptors, Serotonin / biosynthesis
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Receptors, Serotonin / genetics*
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Superior Cervical Ganglion / chemistry
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Up-Regulation / drug effects
Substances
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DNA, Complementary
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Neoplasm Proteins
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Nerve Growth Factors
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Nerve Tissue Proteins
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RNA, Messenger
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RNA, Neoplasm
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Receptors, Serotonin
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RNA-Directed DNA Polymerase