Abstract
Tricyclodecan-9-yl-xanthogenate (compound D609) has recently been used in various cellular systems to specifically inhibit the activity of phosphatidylcholine (PtdCho)-directed phospholipase C (PLC). Here we show that in intact NIH 3T3 fibroblasts, concentrations of D609 (35 to 50 micrograms/ml) which have been used to inhibit PLC activity also significantly inhibit phorbol ester-induced phospholipase D-mediated hydrolysis of both PtdCho and phosphatidylethanolamine (PtdEtn). In addition, in isolated membranes compound D609 also inhibited PLC-mediated PtdEtn hydrolysis. The results indicate that compound D609 cannot be considered as a specific inhibitor of PtdCho-directed PLC activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Bridged-Ring Compounds / pharmacology*
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Cell Membrane / metabolism
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Choline / metabolism
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Enzyme Activation / drug effects
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Hydrolysis
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Kinetics
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Membrane Lipids / metabolism
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Mice
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Norbornanes
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Phosphatidylethanolamines / metabolism*
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Phosphodiesterase Inhibitors / pharmacology*
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Phospholipase D / antagonists & inhibitors
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Phospholipase D / metabolism*
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Thiocarbamates
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Thiones / pharmacology*
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Type C Phospholipases / antagonists & inhibitors
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Type C Phospholipases / metabolism*
Substances
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Bridged-Ring Compounds
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Membrane Lipids
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Norbornanes
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Phosphatidylethanolamines
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Phosphodiesterase Inhibitors
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Thiocarbamates
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Thiones
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tricyclodecane-9-yl-xanthogenate
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Type C Phospholipases
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Phospholipase D
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Choline