Abstract
Adenosine uptake by cultured cerebral endothelium is a carrier-mediated process. The Km value for adenosine uptake is 5.0 microM and the vmax is 1.15 nmol/min/mg cell protein. The uptake system is inhibited by the adenosine analog 2-chloroadenosine at low adenosine concentrations. The results prove the existence of a nucleoside transport system associated with cerebral capillary endothelium.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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2-Chloroadenosine
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Adenosine / analogs & derivatives
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Adenosine / metabolism*
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Adenosine / pharmacology
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Animals
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Biological Transport / drug effects
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Carbon Radioisotopes
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Cell Line
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Cells, Cultured
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Cerebrovascular Circulation* / drug effects
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Dipyridamole / pharmacology
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Endothelium / drug effects
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Endothelium / metabolism
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Kinetics
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Mice
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Papaverine / pharmacology
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Sucrose / metabolism
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Tritium
Substances
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Carbon Radioisotopes
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Tritium
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2-Chloroadenosine
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Sucrose
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Dipyridamole
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Papaverine
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Adenosine