A radioenzymatic method for S-adenosyl-L-methionine determination in biological fluids

Anal Biochem. 1984 Feb;137(1):217-20. doi: 10.1016/0003-2697(84)90373-7.

Abstract

Current methods for the radioenzymatic assay of S-adenosyl-L-methionine (AdoMet) in biological fluids have been modified in order to increase sensitivity. The modified procedure has allowed to measure AdoMet content also in plasma and cerebrospinal fluid where the concentrations have been found to range between 17 and 72 ng/ml in the different animal species.

Publication types

  • Comparative Study

MeSH terms

  • Acetylserotonin O-Methyltransferase*
  • Animals
  • Carbon Radioisotopes
  • Chromatography, High Pressure Liquid
  • Dogs
  • Humans
  • Melatonin
  • Methyltransferases*
  • Rabbits
  • Radiochemistry* / methods
  • Rats
  • S-Adenosylmethionine / analysis*
  • S-Adenosylmethionine / blood
  • S-Adenosylmethionine / cerebrospinal fluid
  • Serotonin / analogs & derivatives
  • Serotonin / chemical synthesis
  • Tritium

Substances

  • Carbon Radioisotopes
  • Tritium
  • Serotonin
  • S-Adenosylmethionine
  • Methyltransferases
  • Acetylserotonin O-Methyltransferase
  • Melatonin
  • N-acetylserotonin