A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers

Anal Biochem. 1983 May;131(1):254-6. doi: 10.1016/0003-2697(83)90163-x.

Abstract

A procedure for the direct solubilization of fibroblast monolayers allowing precise determination of DNA and protein content has been developed. This simple procedure is applicable to the analysis of these macromolecules in fibroblast cultures containing small numbers of cells (less than 1 X 10(5].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Buffers
  • Cells, Cultured
  • DNA / analysis*
  • Fibroblasts / analysis*
  • Humans
  • Indoles
  • Polyethylene Glycols
  • Proteins / analysis*
  • Sodium Hydroxide
  • Solubility
  • Spectrometry, Fluorescence
  • Spectrophotometry

Substances

  • Buffers
  • Indoles
  • Proteins
  • Polyethylene Glycols
  • DAPI
  • Sodium Hydroxide
  • DNA