Abstract
The plasma half-life of beta-galactosidase in rat was about 1.5 min. Ten minutes after in vivo injection, 45% of the enzyme was recovered in liver, with hepatocytes and endothelial cells as the predominant cell types responsible for uptake. In vitro uptake of beta-galactosidase in hepatocytes and nonparenchymal liver cells was saturable, Ca2+-dependent and it could be partly inhibited by mannose or alpha-methyl-mannoside.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium / pharmacology
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Cattle
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Cell Fractionation
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Cell Separation
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Centrifugation, Density Gradient
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Endocytosis
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Galactosidases / metabolism*
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Injections, Intravenous
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Liver / cytology*
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Liver / ultrastructure
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Male
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Mannose-Binding Lectins*
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Rats
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Rats, Inbred Strains
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Receptors, Immunologic / metabolism
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Receptors, Immunologic / physiology
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Testis / enzymology
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beta-Galactosidase / blood
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beta-Galactosidase / metabolism*
Substances
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Mannose-Binding Lectins
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Receptors, Immunologic
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mannose-N-acetylglucosamine receptor
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Galactosidases
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beta-Galactosidase
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Calcium