Increased 13-hydroxyoctadecadienoic acid content in lipopolysaccharide stimulated macrophages

Biochem Biophys Res Commun. 1987 Sep 15;147(2):695-700. doi: 10.1016/0006-291x(87)90986-7.

Abstract

Endotoxin-stimulated mouse peritoneal macrophages were found to contain 13-hydroxyoctadecadienoic acid, which was released upon alkaline hydrolysis of the cells. Compared to untreated cells, incubation with LPS increased the content of 13-hydroxyoctadecadienoic acid in macrophage hydrolysates to about 8-fold. Analysis of the material on chiralphase HPLC revealed that it consisted prevalently of 13(S)-hydroxyoctadecadienoic acid. This indicates its enzymatic origine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Gas Chromatography-Mass Spectrometry
  • Linoleic Acids / metabolism*
  • Lipopolysaccharides / pharmacology*
  • Macrophages / metabolism*
  • Mice
  • Peritoneal Cavity / cytology
  • Salmonella

Substances

  • Linoleic Acids
  • Lipopolysaccharides
  • 13-hydroxy-9,11-octadecadienoic acid