Abstract
Concomitant with the depletion of glutathione content, phorone (250 mg/kg, ip.) produced a marked increase in heme oxygenase activity, biphasic effect on delta-aminolevulinic acid synthetase activity, and slight decreases in cytochrome P-450 content and aminopyrine demethylase activity in the liver of rats. The increase in heme oxygenase activity evoked by phorone was almost completely blocked by pretreatment of rats with actinomycin D and cycloheximide. Phorone was able to produce the changes in these parameters in a dose-dependent manner. Buthionine sulfoximine, a GSH depletor by inhibition of biosynthesis, failed to affect these hepatic parameters.
MeSH terms
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5-Aminolevulinate Synthetase / metabolism*
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Aminopyrine N-Demethylase / metabolism*
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Animals
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Cycloheximide / pharmacology
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Cytochrome P-450 Enzyme System / metabolism*
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Dactinomycin / pharmacology
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Glutathione / antagonists & inhibitors
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Heme / metabolism*
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Heme Oxygenase (Decyclizing) / metabolism*
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Ketones / pharmacology*
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Liver / drug effects
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Liver / metabolism*
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Male
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Microsomes, Liver / enzymology*
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Mitochondria, Liver / enzymology
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Mixed Function Oxygenases / metabolism*
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Rats
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Rats, Inbred Strains
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Solvents
Substances
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Ketones
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Solvents
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Dactinomycin
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Heme
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phorone
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Cytochrome P-450 Enzyme System
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Cycloheximide
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Mixed Function Oxygenases
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Heme Oxygenase (Decyclizing)
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Aminopyrine N-Demethylase
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5-Aminolevulinate Synthetase
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Glutathione