Neutrophil LTA4 hydrolases and leukotriene B4 receptors: effects of leukotriene epoxides and their enzymatic products

Prostaglandins Leukot Med. 1986 Aug;23(2-3):167-71. doi: 10.1016/0262-1746(86)90181-2.

Abstract

A selection of inhibitors of rat and human neutrophil LTA4 hydrolases have been studied in vitro using partially purified enzymes. 5(S)trans 5,6 oxido-7,9-trans-11-cis-eicosatrienoic acid (LTA3) and 5(S)trans 5,6 oxido, 7,9-trans, 11,14,17-cis-eicosapentaenoic acid (LTA5) have been shown to inhibit neutrophil LTA4 hydrolases in a time-dependent manner. The products of hydrolysis of LTA3, LTA4 and LTA5 by human and rat neutrophil LTA4 hydrolase have been shown to displace [3H] LTB4 binding to human and rat neutrophil membranes. The order of displacement of [3H] LTB4 is LTB4 = LTB3 greater than LTB5 and this correlated well with their biological potencies for enhancement of neutrophil aggregation and chemokinesis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Arachidonic Acids / metabolism
  • Arachidonic Acids / pharmacology*
  • Cell Membrane / metabolism
  • Eicosapentaenoic Acid / analogs & derivatives
  • Eicosapentaenoic Acid / metabolism
  • Eicosapentaenoic Acid / pharmacology
  • Epoxide Hydrolases / antagonists & inhibitors
  • Epoxide Hydrolases / physiology*
  • Humans
  • Hydrolysis
  • Leukotriene A4
  • Leukotriene B4 / metabolism*
  • Neutrophils / enzymology*
  • Neutrophils / metabolism
  • Neutrophils / ultrastructure
  • Rats
  • Receptors, Immunologic / physiology*
  • Receptors, Leukotriene B4
  • Time Factors

Substances

  • Arachidonic Acids
  • Leukotriene A4
  • Receptors, Immunologic
  • Receptors, Leukotriene B4
  • Leukotriene B4
  • leukotriene B5
  • leukotriene B3
  • Eicosapentaenoic Acid
  • Epoxide Hydrolases
  • leukotriene A4 hydrolase