Assay of cyclic nucleotide phosphodiesterase using radiolabeled and fluorescent substrates

Methods Enzymol. 1988:159:457-70. doi: 10.1016/0076-6879(88)59045-6.

Authors

R L Kincaid, V C Manganiello

PMID: 2842611
DOI: 10.1016/0076-6879(88)59045-6

No abstract available

MeSH terms

3',5'-Cyclic-AMP Phosphodiesterases / metabolism*
3',5'-Cyclic-GMP Phosphodiesterases / metabolism*
Cyclic AMP / metabolism
Cyclic GMP / metabolism
Indicators and Reagents
Kinetics
Radioisotope Dilution Technique
Spectrometry, Fluorescence / methods
Tritium

Substances

Indicators and Reagents
Tritium
Cyclic AMP
3',5'-Cyclic-AMP Phosphodiesterases
3',5'-Cyclic-GMP Phosphodiesterases
Cyclic GMP