Prednisolone at concentrations of 10(-5) to 10(-8) mol/l, added to 3-day (day D + 2) tissue cultures of newborn rat myogenic cells at the time myoblasts are beginning to fuse, increases the level of myotube acetylcholine receptor expression at the 8th day (day D + 7) of culture. This effect is associated with increases in the number and size of the formed myotubes, not with a changed affinity of the receptor for its ligand, and is very probably mediated by one or more extracellular proteins the synthesis of which is induced early by the presence of prednisolone.