Mass changes of inositol(1,4,5)trisphosphate in trachealis muscle following agonist stimulation

Eur J Pharmacol. 1989 May 30;164(3):587-90. doi: 10.1016/0014-2999(89)90269-0.

Abstract

Agonist-stimulated changes in D-Ins(1,4,5)P3 has been examined in tracheal smooth muscle using a specific radioreceptor assay utilizing the binding of [3H] or [32P]Ins(1,4,5)P3 to a bovine adrenal cortex preparation. Carbachol produced a maximal increase in Ins(1,4,5)P3 concentration after 5 s (basal: 12.9 +/- 0.8; stimulated: 27 +/- 1.5 pmol/mg protein) with an EC50 of 6.7 +/- 0.8 microM. With longer stimulation periods Ins(1,4,5)P3 rapidly returned to basal values by 30 s. This is the first report of mass measurements of Ins(1,4,5)P3 in smooth muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Atropine / pharmacology
  • Calcium Channels*
  • Carbachol / pharmacology
  • Cattle
  • In Vitro Techniques
  • Inositol 1,4,5-Trisphosphate
  • Inositol 1,4,5-Trisphosphate Receptors
  • Inositol Phosphates / metabolism*
  • Muscle, Smooth / drug effects
  • Muscle, Smooth / metabolism*
  • Parasympathomimetics / pharmacology*
  • Receptors, Cell Surface / metabolism
  • Receptors, Cytoplasmic and Nuclear*
  • Sugar Phosphates / metabolism*
  • Trachea / drug effects
  • Trachea / metabolism

Substances

  • Calcium Channels
  • Inositol 1,4,5-Trisphosphate Receptors
  • Inositol Phosphates
  • Parasympathomimetics
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • Sugar Phosphates
  • Atropine
  • Inositol 1,4,5-Trisphosphate
  • Carbachol