Abstract
Agonist-stimulated changes in D-Ins(1,4,5)P3 has been examined in tracheal smooth muscle using a specific radioreceptor assay utilizing the binding of [3H] or [32P]Ins(1,4,5)P3 to a bovine adrenal cortex preparation. Carbachol produced a maximal increase in Ins(1,4,5)P3 concentration after 5 s (basal: 12.9 +/- 0.8; stimulated: 27 +/- 1.5 pmol/mg protein) with an EC50 of 6.7 +/- 0.8 microM. With longer stimulation periods Ins(1,4,5)P3 rapidly returned to basal values by 30 s. This is the first report of mass measurements of Ins(1,4,5)P3 in smooth muscle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Atropine / pharmacology
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Calcium Channels*
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Carbachol / pharmacology
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Cattle
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In Vitro Techniques
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Inositol 1,4,5-Trisphosphate
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Inositol 1,4,5-Trisphosphate Receptors
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Inositol Phosphates / metabolism*
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Muscle, Smooth / drug effects
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Muscle, Smooth / metabolism*
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Parasympathomimetics / pharmacology*
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Receptors, Cell Surface / metabolism
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Receptors, Cytoplasmic and Nuclear*
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Sugar Phosphates / metabolism*
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Trachea / drug effects
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Trachea / metabolism
Substances
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Calcium Channels
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Inositol 1,4,5-Trisphosphate Receptors
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Inositol Phosphates
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Parasympathomimetics
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Receptors, Cell Surface
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Receptors, Cytoplasmic and Nuclear
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Sugar Phosphates
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Atropine
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Inositol 1,4,5-Trisphosphate
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Carbachol