Abstract
In this study, the effects of benzo[a]pyrene (BP), 3-methylcholanthrene (3MC) and tetrachlorodibenzofuran (TCDBF) on the expression of the cytochrome P450IA1 gene in the rat hepatoma cell line H4IIE were examined. The initial rate of increase in the steady-state concentration of the mRNA for this gene was similar with each of these inducers; however, the elevated level of this mRNA was more sustained after TCDBF treatment. Nuclear run-off assays suggested that the elevated level of the mRNA was caused principally by an effect upon transcription.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Benzo(a)pyrene / pharmacology*
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Benzofurans / pharmacology*
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Cytochrome P-450 Enzyme System / biosynthesis*
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Dioxins / pharmacology*
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Enzyme Induction / drug effects
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Gene Expression Regulation, Enzymologic / drug effects*
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Isoenzymes / biosynthesis*
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Liver Neoplasms, Experimental / enzymology*
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Methylcholanthrene / pharmacology*
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Polychlorinated Dibenzodioxins / pharmacology*
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Rats
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Tumor Cells, Cultured / drug effects
Substances
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Benzofurans
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Dioxins
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Isoenzymes
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Polychlorinated Dibenzodioxins
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Benzo(a)pyrene
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Methylcholanthrene
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Cytochrome P-450 Enzyme System
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2,3,7,8-tetrachlorodibenzofuran