Induction of cytochrome P450IA1 in rat hepatoma cell by polycyclic hydrocarbons and a dioxin

Biochem Pharmacol. 1990 Sep 15;40(6):1399-403. doi: 10.1016/0006-2952(90)90409-e.

Abstract

In this study, the effects of benzo[a]pyrene (BP), 3-methylcholanthrene (3MC) and tetrachlorodibenzofuran (TCDBF) on the expression of the cytochrome P450IA1 gene in the rat hepatoma cell line H4IIE were examined. The initial rate of increase in the steady-state concentration of the mRNA for this gene was similar with each of these inducers; however, the elevated level of this mRNA was more sustained after TCDBF treatment. Nuclear run-off assays suggested that the elevated level of the mRNA was caused principally by an effect upon transcription.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Benzo(a)pyrene / pharmacology*
  • Benzofurans / pharmacology*
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Dioxins / pharmacology*
  • Enzyme Induction / drug effects
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Isoenzymes / biosynthesis*
  • Liver Neoplasms, Experimental / enzymology*
  • Methylcholanthrene / pharmacology*
  • Polychlorinated Dibenzodioxins / pharmacology*
  • Rats
  • Tumor Cells, Cultured / drug effects

Substances

  • Benzofurans
  • Dioxins
  • Isoenzymes
  • Polychlorinated Dibenzodioxins
  • Benzo(a)pyrene
  • Methylcholanthrene
  • Cytochrome P-450 Enzyme System
  • 2,3,7,8-tetrachlorodibenzofuran