High-speed separations of DNA sequencing reactions by capillary electrophoresis

H Drossman; J A Luckey; A J Kostichka; J D'Cunha; L M Smith

doi:10.1021/ac00208a003

High-speed separations of DNA sequencing reactions by capillary electrophoresis

Anal Chem. 1990 May 1;62(9):900-3. doi: 10.1021/ac00208a003.

Authors

H Drossman¹, J A Luckey, A J Kostichka, J D'Cunha, L M Smith

Affiliation

¹ Department of Chemistry, University of Wisconsin, Madison 53706.

PMID: 2363514
DOI: 10.1021/ac00208a003

Abstract

Fluorescently labeled DNA fragments generated in enzymatic sequencing reactions are rapidly separated by capillary gel electrophoresis and detected at attomole levels within the gel-filled capillary. The application of this technology to automated DNA sequence analysis may permit the development of a second generation automated sequencer capable of efficient and cost-effective sequence analysis on the genomic scale.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
DNA / analysis*
Electrophoresis
Molecular Sequence Data
Spectrometry, Fluorescence

Substances

DNA

Grants and funding

GM 42366/GM/NIGMS NIH HHS/United States