Abstract
In vitro radioligand assay revealed interaction of afobazole with sigma(1)-receptors (Ki=5.9x10(-6) M). Translocation of sigma(1)-receptors from the endoplasmic reticulum to the outer membrane was demonstrated by confocal microscopy. Experiments were performed on the model of HT-22 immortalized hippocampal cells after incubation with afobazole in a concentration of 10(-8) M.
MeSH terms
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Animals
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Anti-Anxiety Agents / pharmacology*
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Benzimidazoles / pharmacology*
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Cell Line, Transformed
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Fluorescent Antibody Technique
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Hippocampus / cytology
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Hippocampus / drug effects
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Humans
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Jurkat Cells
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Mice
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Morpholines / pharmacology*
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Neuroprotective Agents / pharmacology*
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Radioligand Assay
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Receptors, sigma / drug effects*
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Sigma-1 Receptor
Substances
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2-((2-morpholino)ethylthio)-5-ethoxybenzimidazole
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Anti-Anxiety Agents
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Benzimidazoles
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Morpholines
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Neuroprotective Agents
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Receptors, sigma