Abstract
Peroxisome proliferator activated receptor gamma (PPARgamma) plays a critical role in controlling immune and inflammatory responses. However, its effect on pulpal inflammation has not been clarified. The purpose of this study was to determine the anti-inflammatory effect of PPARgamma on pulpal inflammation. Human dental pulp cells treated with lipopolysaccharide exhibited elevated levels of matrix metalloproteinase-2 (MMP-2), MMP-9, intracellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, when treated with rosiglitazone (PPARgamma agonist) or adenoviral PPARgamma in same culture system, the expression of ICAM-1 and VCAM-1 was markedly inhibited along with decreased secretion of MMPs. In addition, the coadministration of GW9662 (PPARgamma antagonist) and rosiglitazone blocked the inhibition of MMP-2, MMP-9, ICAM-1, and VCAM-1. These results suggest that PPARgamma decreased the production of MMPs, ICAM-1, and VCAM-1 and might offer a possible attempt of using it as one of anti-inflammatory modulators in a pulpal inflammation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anilides / pharmacology
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Anti-Inflammatory Agents / pharmacology*
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Blotting, Western
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Cells, Cultured
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Dental Pulp / cytology
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Dental Pulp / metabolism*
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Escherichia coli / chemistry
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Fibroblasts / metabolism
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Humans
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Inflammation Mediators / metabolism*
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Intercellular Adhesion Molecule-1 / biosynthesis
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Lipopolysaccharides
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Matrix Metalloproteinase 2 / biosynthesis
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Matrix Metalloproteinase 9 / biosynthesis
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Matrix Metalloproteinase Inhibitors
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PPAR gamma / agonists
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PPAR gamma / antagonists & inhibitors
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PPAR gamma / pharmacology
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PPAR gamma / physiology*
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Pulpitis / metabolism*
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Recombinant Proteins / pharmacology
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Rosiglitazone
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Thiazolidinediones / pharmacology
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Vascular Cell Adhesion Molecule-1 / biosynthesis
Substances
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2-chloro-5-nitrobenzanilide
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Anilides
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Anti-Inflammatory Agents
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Inflammation Mediators
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Lipopolysaccharides
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Matrix Metalloproteinase Inhibitors
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PPAR gamma
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Recombinant Proteins
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Thiazolidinediones
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Vascular Cell Adhesion Molecule-1
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Rosiglitazone
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Intercellular Adhesion Molecule-1
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9