Isolation of IgGT from hyperimmune horse anti-snake venom serum: its protective ability

I Fernandes; H A Takehara; I Mota

doi:10.1016/0041-0101(91)90124-a

Isolation of IgGT from hyperimmune horse anti-snake venom serum: its protective ability

Toxicon. 1991;29(11):1373-9. doi: 10.1016/0041-0101(91)90124-a.

Authors

I Fernandes¹, H A Takehara, I Mota

Affiliation

¹ Immunology Research and Training Center Otto G. Bier, Instituto Butantan, São Paulo, Brazil.

PMID: 1814013
DOI: 10.1016/0041-0101(91)90124-a

Abstract

Hyperimmune horse anti-bothropic serum, used in serum therapy, was analyzed for its IgGT content and protective ability. IgGT was isolated through a combination of salt-mediated hydrophobic chromatography and protein A affinity chromatography. The chromatographic fractions obtained were analyzed with regard to their isotype content and protective ability. The results suggest that the protective ability of hyperimmune anti-venom serum is located mainly in the IgGT subclass.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antivenins / isolation & purification*
Chromatography, Affinity
Crotalid Venoms / immunology*
Crotalid Venoms / toxicity
Horses / immunology*
Immunization
Immunoglobulin G / isolation & purification*
Immunoglobulin Isotypes / isolation & purification
Lethal Dose 50
Male
Mice
Rabbits
Staphylococcal Protein A / immunology

Substances

Antivenins
Crotalid Venoms
Immunoglobulin G
Immunoglobulin Isotypes
Staphylococcal Protein A