Abstract
A robust method for the routine quantitation of a selected group of urinary eicosanoid metabolites of interest to cardiovascular research in human and mouse is described and discussed. Included are the addition of stable isotope-labeled internal standards, solid phase extraction, and quantitation by liquid chromatography/tandem mass spectrometry using selected reaction monitoring (SRM) techniques.
MeSH terms
-
Animals
-
Biomarkers / metabolism
-
Biomarkers / urine
-
Cardiovascular Diseases / enzymology
-
Cardiovascular Diseases / urine
-
Cardiovascular System / metabolism*
-
Chromatography, High Pressure Liquid / methods
-
Eicosanoids / metabolism*
-
Eicosanoids / standards
-
Eicosanoids / urine
-
Humans
-
Mice
-
Prostaglandin-Endoperoxide Synthases / metabolism*
-
Reference Standards
-
Tandem Mass Spectrometry / methods
Substances
-
Biomarkers
-
Eicosanoids
-
Prostaglandin-Endoperoxide Synthases