Abstract
Human recombinant interleukin 6 (rhIL-6) caused a dose dependent decrease in the phenobarbital induction of benzyloxyresorufin O-deethylase activity in cultured rat hepatocytes. Decreased enzymatic activity was associated with a decrease in the amount of immunoreactive P-450IIB1/2. rhIL-6 also prevented the PB-induced increase in the steady state level of P-450IIB mRNA. These results suggest that altered P-450 levels observed in vivo during the acute phase reaction may be due to interleukin 6.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cells, Cultured
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Cytochrome P-450 CYP2B1
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Cytochrome P-450 Enzyme System / biosynthesis*
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Cytochrome P-450 Enzyme System / genetics
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Enzyme Induction
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Exons
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Interleukin-6 / pharmacology*
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Kinetics
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Liver / drug effects
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Liver / enzymology*
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Male
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Molecular Sequence Data
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Oligonucleotide Probes
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Oxidoreductases / biosynthesis*
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Oxidoreductases / genetics
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Phenobarbital / pharmacology*
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RNA / genetics
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RNA / isolation & purification
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Rats
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Rats, Inbred F344
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Recombinant Proteins / pharmacology
Substances
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Interleukin-6
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Oligonucleotide Probes
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Recombinant Proteins
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RNA
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Cytochrome P-450 Enzyme System
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Oxidoreductases
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Cytochrome P-450 CYP2B1
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Phenobarbital