Abstract
Human atrial natriuretic peptide [ANF(1-28)] contains five arginine residues and carries an overall positive change of four. It was hypothesized that atrial peptides may induce mast cell histamine release. In vitro, three atrial peptides [ANF(1-28), (3-28) and (5-28)] were demonstrated to induce dose-dependent histamine release from isolated rat peritoneal mast cells. In vivo, ANF(3-28) produced a dose-dependent increase in rat skin permeability which was blocked by antagonists of histamine and serotonin. The results indicate atrial peptides are capable of inducing mast cell degranulation in a manner similar to that described for other positively charged peptides.
MeSH terms
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Amino Acid Sequence
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Animals
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Atrial Natriuretic Factor / administration & dosage
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Atrial Natriuretic Factor / pharmacology*
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Dose-Response Relationship, Drug
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Histamine Antagonists / pharmacology
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Histamine Release / drug effects*
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Injections, Intradermal
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Male
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Mast Cells / drug effects*
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Molecular Sequence Data
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Peptide Fragments / administration & dosage
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Peptide Fragments / pharmacology*
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Rats
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Rats, Inbred Strains
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Serotonin Antagonists / pharmacology
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Stimulation, Chemical
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Structure-Activity Relationship
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Substance P / pharmacology
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p-Methoxy-N-methylphenethylamine / pharmacology
Substances
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Histamine Antagonists
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Peptide Fragments
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Serotonin Antagonists
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atrial natriuretic factor (3-28)
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Substance P
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p-Methoxy-N-methylphenethylamine
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Atrial Natriuretic Factor
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atrial natriuretic factor (5-28)