Abstract
A cDNA of rat brain encoding the GABAA receptor alpha 4 subunit has been cloned. Recombinant receptors composed of alpha 4, beta 2 and gamma 2 subunit bind with high affinity the GABA agonist [3H]muscimol and the benzodiazepine 'alcohol antagonist' [3H]Ro 15-4513, but fail to bind benzodiazepine agonists. The alpha 4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABAA receptors. The alpha 4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Affinity Labels / metabolism
-
Amino Acid Sequence
-
Animals
-
Azides / metabolism
-
Base Sequence
-
Benzodiazepines / metabolism
-
Binding, Competitive
-
Brain / metabolism
-
Cloning, Molecular
-
Kinetics
-
Macromolecular Substances
-
Molecular Sequence Data
-
Muscimol / metabolism
-
Oligodeoxyribonucleotides
-
Organ Specificity
-
RNA, Messenger / analysis
-
RNA, Messenger / genetics
-
Rats
-
Receptors, GABA-A / analysis
-
Receptors, GABA-A / genetics*
-
Receptors, GABA-A / metabolism
-
Recombinant Proteins / analysis
-
Recombinant Proteins / metabolism
Substances
-
Affinity Labels
-
Azides
-
Gabra4 protein, rat
-
Macromolecular Substances
-
Oligodeoxyribonucleotides
-
RNA, Messenger
-
Receptors, GABA-A
-
Recombinant Proteins
-
Benzodiazepines
-
Muscimol
-
Ro 15-4513
Associated data
-
GENBANK/S55539
-
GENBANK/S55855
-
GENBANK/S55864
-
GENBANK/S55933
-
GENBANK/X58799
-
GENBANK/X58800
-
GENBANK/X58801
-
GENBANK/X58802
-
GENBANK/X58803
-
GENBANK/X58995