Abstract
Cocaine is among the most reinforcing of all drugs of abuse, yet no effective pharmacotherapy is available. Herein, we report the development and characterization of phage-displayed cocaine esterases with pharmacologically relevant kinetic parameters (kcat/Km approximately 104 M-1 s-1).
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacteriophages / chemistry*
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Bacteriophages / enzymology*
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Bacteriophages / genetics
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Carboxylic Ester Hydrolases / biosynthesis*
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Carboxylic Ester Hydrolases / chemistry*
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Carboxylic Ester Hydrolases / genetics
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Cocaine / chemistry*
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Escherichia coli / genetics
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Hydrolysis
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Kinetics
Substances
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Bacterial Proteins
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Carboxylic Ester Hydrolases
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cocaine esterase
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Cocaine